单核细胞增生性李斯特氏菌种、株随机扩增DNA多态性研究  

Listeria monocytogenes species and strains on DNA polymorphisms

作　　者：金莉莉[1] 王秋雨[1] 王海鹏[2] 

机构地区：[1]辽宁大学生命科学系,沈阳110036 [2]中国医科大学病原生物学教研室

出　　处：《中国人兽共患病杂志》2003年第6期38-41,共4页Chinese Journal of Zoonoses

基　　金：辽宁省教委科研基金资助项目

摘　　要：目的　分析单核细胞增生性李斯特氏菌种、株基因组DNA多态性 ,为其分型、鉴定提供理论依据。方法　应用随机扩增多态性DNA技术对李斯特氏菌基因组DNA进行扩增 ,根据DNA指纹图谱分析单核细胞增生性李斯特氏菌种、株的遗传多样性 ,同时构建相似性系数矩阵和树状图。结果　不同序列的随机引物扩增的RAPD图谱的多态性程度不同 ,其中单一引物P-7与复合引物P-1P-3 、P-4P-9扩增产生的指纹图谱对单核细胞增生性李斯特氏菌种、株均具有良好的区分能力。由该 3种引物扩增的RAPD图谱计算出的相似性系数矩阵及由此构建的聚类树状图均能得到李斯特氏菌的系统发育关系。结论　RAPD技术可用于李斯特氏菌的快速鉴定。Aim To analysis the polymorphism of the genomic DNA from Listeria monocytogenes,and provide the theoretical basis for its typing and identification.Methods Genomic DNA of species and strains from Listeria spp.was amplified by the technique of random amplified polymorphic DNA(RAPD),and its genetic polymorphism,proximity matrix and cluster dendrogram were made out according to the RAPD fingerprinting.Result The polymorphic degrees of RAPD fingerprinting obtained with different primers were different,and the single primer P -3,the composed primer P -1P -3 and P -4P -9 could produce the fingerprintings which had a good ability to discriminate the species and strains of Listeria monocytogenes.The relationship of systematic development of Listeria spp.could be analysised by the proximity matrix and cluster dendrogram got from the fingerprintings of these three primers.Conclusion The typing and identification of the species and strains of Listeria monocytogenes can be resolved by the technique of RAPD.

关 键 词：单核细胞增生性李斯特氏菌 菌种 菌株 基因扩增 基因多态性 DNA指纹图谱 

分 类 号：R378.99[医药卫生—病原生物学] R394[医药卫生—基础医学]