肺肿瘤细胞膜表面糖复合物与组织类型关系的研究  

The relationship between glycoconjugate expression on cell surface and histological classification of pulmonary tumors

作　　者：王晓玫[1] 成志强[1] 单军[2] 陈灼怀[1] 

机构地区：[1]暨南大学医学院附属二院深圳市人民医院病理科,518020 [2]暨南大学医学院附属二院深圳市人民医院CT室,518020

出　　处：《中国肺癌杂志》2003年第2期111-115,共5页Chinese Journal of Lung Cancer

基　　金：深圳市卫生科技计划重点攻关项目 (No.1 9980 50 1 1 );国家人事部科技择优项目 (1 9990 1 2 2 )~~

摘　　要：目的　探讨肺肿瘤多阶段进展中细胞膜表面糖复合物与组织类型间的关系 ,为肺肿瘤的良恶性诊断提供客观依据。方法　乙酰胆蒽诱导小鼠得到不同组织学类型肺部病变 2 92个 ,其中良性 (增生性病变、肺泡细胞腺瘤、乳头状腺瘤 )病变 2 37个 ,恶性病变 (乳头状腺癌 ) 5 5个。检测Maclurapomiferaagglutinin(MPA)、花生凝集素 (Arachishypogeaagglutinin ,PNA)、蓖麻凝集素 (Ricinuscommunisagglutinin ,RCA)和麦胚凝集素 (wheatgermagglutinin ,WGA)辣根过氧化物酶凝集素与正常呼吸道上皮及各型病变的亲和性。 结果　增生性病变、肺泡细胞腺瘤与 4种凝集素均保持高度亲和性 ,乳头状病变尤其是乳头状腺癌细胞则基本失去了对MPA、PNA和RCA的亲和性 ,仅保留与WGA的结合力。在良、恶性病变的比较中 ,MPA、PNA和RCA的亲和性均有显著性差异 (χ2 =46.89,P <0 .0 1;χ2 =36.77,P <0 .0 1;χ2 =5 2 .87,P <0 .0 1) ,WGA与良、恶性病变均有较强的亲和性 (χ2 =0 .0 9,P >0 .0 5 )。结论　由于不同类型肺部病变细胞膜表面糖复合物残基的差异 ,良、恶性病变与MPA、PNA和RCA的亲和性明显不同。检测细胞与不同凝集素的亲和反应 ,有助于肺部良。Objective To investigate the relationship between progressive development of mouse pulmonary tumors and expression of cell surface saccharide and to provide a theoretical basis for diagnosis of pulmonary benign and malignant lesions. Methods A/J strain mice at 5 weeks of age were treated intraperitoneally with 20 methylcholanthrene, and 292 pulmonary lesions including 237 benign lesions (hyperplasia, alveolar adenoma, and papillary adenoma) and 55 malignant tumors (papillary adenocarcinoma) were obtained. The binding affinities of cells in normal respiratory epithelia and various proliferative lesions to four peroxidase conjugated lectins, Maclura pomifera agglutinin (MPA), Arachis hypogea agglutinin (PNA), Ricinus communis agglutinin (RCA), and wheat germ agglutinin (WGA) were examined. Results Cells of hyperplasias and alveolar adenomas showed fairly strong affinities to all the lectins. However, most of papillary adenoma cells and papillary adenocarcinoma cells lost their binding affinities to MPA, PNA, and RCA, but not to WGA. Between the benign and malignant lesions, there were significant differences in binding affinities of cells to MPA (χ 2 =46.89,P<0.01), PNA (χ 2 =36.77,P<0.01) and RCA (χ 2=52.87,P<0.01), but not to WGA (χ 2=0.09,P>0.05).Conclusion According to the different complex glycoconjugates on cell surface of various pulmonary lesions, the binding affinities to MPA, PNA and RCA are quite different between the benign and malignant lesions. The detection of bindings is helpful to the differential diagnosis of the pulmonary benign and malignant lesions.

关 键 词：肺肿瘤 细胞膜 糖复合物 乙酰胆蒽 凝集素 小鼠 

分 类 号：R734.2[医药卫生—肿瘤]