胃黏膜线粒体DNA不稳定与白细胞介素8活性的关系  

Relationship between mitochondrial DNA instability and interleukin-8 activity in gastric mucosa

作　　者：凌贤龙[1] 房殿春[1] 汪荣泉[1] 杨仕明[1] 罗元辉[1] 

机构地区：[1]第三军医大学西南医院全军消化专科中心,重庆400038

出　　处：《中华病理学杂志》2003年第1期35-37,共3页Chinese Journal of Pathology

基　　金：国家自然科学基金资助项目 ( 30 0 70 0 43);全军"十.五"科研基金重点项目 ( 0 1Z0 75 )

摘　　要：目的　评价胃黏膜线粒体DNA不稳定 (mtMSI)与组织白细胞介素 8(IL 8)活性的关系。方法　采用聚合酶链反应 单链构象多态性分析 (PCR SSCP)方法检测mtMSI;组织IL 8含量测定采用ELISA方法。结果　 3 0例胃癌检出mtMSI 11例 ( 3 6 7% ) ,15例肠化胃黏膜组织中有 2例为mtMSI,10例异型增生黏膜组织有 2例为mtMSI,10例萎缩性胃炎黏膜组织有 1例检出mtMSI。胃黏膜细胞IL 8水平在mtMSI阳性组 [( 76 8± 3 8)pg/mg]显著高于mtMSI阴性组 [( 48 3± 3 6)pg/mg ,P <0 0 5 ]。结论　胃黏膜细胞mtMSI可能与IL 8释放有关 。Objective To evaluate the relationship between mitochondrial DNA instability (mtMSI ) and interleukin-8 (IL-8) activity in gastric mucosa of various lesions. Methods IL-8 level in gastric mucosa was assayed using ELISA method. The mtMSI was detected by PCR-SSCP techniques. Results mtMSI was observed in 11 out of 30(36.7%) gastric cancers, 2 of 15(13.3%) intestinal metaplasia, 2 of 10 dysplasia and 1 of 10 chronic atrophic gastritis. IL-8 level in mtMSI+ group [(76.8±3.8) pg/mg] was significantly higher than that in mtMSI- group [(48.3±3.6) pg/mg,P<0.05]. Conclusion mtMSI closely correlates with IL-8 level in gastric mucosa and is involved in gastric carcinogenesis.

关 键 词：胃黏膜 线粒体 DNA 白细胞介素8 PCR-SSCP ELISA 胃癌 氧自由基 

分 类 号：R730.2[医药卫生—肿瘤]