鸡源致病性大肠杆菌1型菌毛pilA基因的PCR扩增、克隆及序列分析  

The PCR and Sequence Analyse of PilA Gene of Type 1 Pili of Pathogenic Escherichia Coli Isolated from Chick

作　　者：叶如俊[1] 王红宁[1] 谭炳乾[1] 

机构地区：[1]四川农业大学动物科技学院,雅安625014

出　　处：《畜牧兽医学报》2004年第1期83-88,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：四川"十五"畜牧重点攻关项目资助(1999 2004)

摘　　要：研究选择从四川规模化鸡场分离鉴定的5株优势血清型鸡源致病性大肠杆菌代表株(O89,O119,O141,O127)的1型菌毛pilA基因的PCR扩增片段分别定向克隆到pUC18质粒的多克隆位点,并转化到DH5α株大肠杆菌载体菌中,对目的基因的序列测定结果表明:5个克隆片段均含有1型菌毛pilA基因的全序列,全长459bp,编码信号肽和结构蛋白。用生物软件(DNASTAR)对9株大肠杆菌1型菌毛pilA基因序列、氨基酸序列、菌毛蛋白质二级结构预测及抗原性进行了分析比较,结果显示:鸡源致病性大肠杆菌1型菌毛间具有同源性,1型菌毛间存在一定的相同抗原位点。In this paper,five amplified fragment of dominant serotype strains(O_(89),O_(119),O_(141),O_(127))in sichuan province of china were cloned and sequenced respectively.The results showed that the coding region is 459 bp in size.The pilA gene sequence,amino acid sequence,secondry sructure and antigen characteristic were analyzed by DNASTAR,a kind of bio-software.The result showed that there had homology in avian pathogenic E.coli.There had some common antigen side in type 1 pili of avian pathogenic E.coli.

关 键 词：鸡 致病性大肠杆菌 1型菌毛 PILA基因 PCR扩增 克隆 序列分析 

分 类 号：S858.31[农业科学—临床兽医学]