IRM-2近交系小鼠肿瘤模型的建立  

Establishment of Tumor Models in inbred Strain IRM-2 Mouse

作　　者：王月英[1] 周继文[1] 穆传杰[1] 岳井银[1] 刘鹏[1] 吴红英[1] 王汝勤[1] 

机构地区：[1]中国医学科学院中国协和医科大学放射医学研究所,天津300192

出　　处：《中国比较医学杂志》2003年第4期246-248,共3页Chinese Journal of Comparative Medicine

基　　金：国家"九五"重点项目 ( 96-A2 3 -0 6-0 4)

摘　　要：目的　建立IRM— 2小鼠自发性肿瘤模型和移植性肿瘤模型 ,为肿瘤研究提供实验模型。方法　采用瘤块接种法及悬液接种法。 (1)无菌环境取 2mm3瘤块 ,接种于小鼠腹股沟皮下。 (2 )将瘤块研磨 ,加生理盐水稀释至 1× 10 7 ml,取细胞悬液 0 2ml(含 1× 10 6 细胞 )注入腋窝皮下 ,观察肿瘤生长情况。结果　接种恶性淋巴瘤连续传 70代 ,成瘤率 10 0 % ,移植津白Ⅱ号高乳腺癌瘤株连续 4 5代、移植T 739肺腺癌瘤株 5代 ,成瘤率均为10 0 % ,且生长稳定 ,传代周期固定。结论　该自发性恶性淋巴瘤具有较强的特异性 ,证明该模型是成立的。Objective To establish spontaneous and transplanting tumor models in the radiation resistant IRM-2 inbred mouse.Methods 1.The spontaneous lymphoma tissues from IRM-2 mouse were cut into several fragments sized 2.0 mm3 then implanted subcutaneously into inguen of IRM-2 mouse, 2.Breast cancer tissues from Jin BaiⅡ mouse and T-739 lung adenocarcinoma tissues were pounded and suspended in physiological saline, then cell suspension was 0.2 ml (1×106 cells ) injected subcutaneously into axilla of IRM-2 mouse. The frequency of tumorgenesis and survival day of bearing tumor animals were examined. Results The spontaneous malignant lymphoma were implanted and passed 70 times(968 mice), the T-739 lung adenocarcinoma 45 times (245 mice), and the breast cancer 5 times (50 mice). The frequencies of tumorgenesis were 100 % for all three types of tumor, The average survival days of animals bearing lymphoma, T-739 lung adenocarcinoma and breast cancer were (26.0±1.6)d, (25.1±1.9)d and (25.5±2.8)d respectively. Conclusion The results showed that tumor models established in IRM-2 inbred mouse were with suceess.

关 键 词：小鼠模型 淋巴瘤 乳腺肿瘤 肺肿瘤 治疗 IRM-2 

分 类 号：R73[医药卫生—肿瘤] R-332[医药卫生—临床医学]