免疫共沉淀法用于肿瘤异质性研究的可行性分析  

Co-immunoprecipitation used as a new method to study on tumor heterogeneity

作　　者：于莉娜[1] 钟雪云[1] 陈运贤[2] 刘致中[1] 秦艳芳[1] 王冰[1] 杜彬[1] 

机构地区：[1]暨南大学医学院病理学教研室,广东广州510632 [2]中山大学附属第一医院血液科,广东广州510080

出　　处：《免疫学杂志》2004年第1期58-60,64,共4页Immunological Journal

基　　金：国家"973"重大基础研究项目 (2 0 0 2ccc0 0 4 0 0 );广东省科技攻关项目 (2 0 0 3B30 1 0 )

摘　　要：目的　探讨免疫共沉淀法在肿瘤异质性蛋白质分子机制研究中的可行性 ,为肿瘤异质性研究建立一种新的方法。方法　用免疫共沉淀法研究来自同一遗传背景的 2株人类胶质瘤细胞亚系 (SWOZ1和SWOZ2 )中鼠双微粒体基因 2 (Mu rinedoubleminute 2 ,MDM2 )相关蛋白表达的异质性。结果　在Mr370 0 0左右处可清楚看到有一蛋白条带SWOZ2明显深染于SWOZ1,提示在MDM2传导通路上此种Mr 在 370 0 0左右的蛋白可能与 2株亚系表现出的不同生物学行为有关。Objective To explore a novel methodology for tumor heterogeneity research, we reveal the feasibility of using co immunoprecipitation in the protein molecular mechanism of tumor heterogeneity. Methods To disclose the difference between the two Glioma cell sublines SWOZ1 and SWOZ2 by using co immunoprecipitation method. Results There is an obvious difference between SWOZ1 and SWOZ2 that is in the protein molecule size of 37 000. This result indicates this protein relates to the different biological characters of those two sub lines in the MDM2 transmit channel. Conclusion Co immunoprecipitation is an excellent method for the research of protein molecular mechanism of tumor heterogeneity.

关 键 词：免疫共沉淀 异质性 鼠双微粒体基因-2 

分 类 号：R73-3[医药卫生—肿瘤]