尿脱落细胞端粒酶检测在膀胱癌诊断中的意义  

Significance of determination of telomerase activity in exfoliated cells in urine for the diagnosis of bladder carcinoma

作　　者：曹明耀[1] 李亚义 

机构地区：[1]河北工程学院医学部,邯郸市056029 [2]山西省长治市惠丰医院

出　　处：《邯郸医学高等专科学校学报》2004年第2期83-84,共2页Journal of Handan Medical College

摘　　要：目的　探讨检测自排尿脱落细胞端粒酶活性在膀胱癌早期诊断中的意义。方法　应用TRAP -PCR-ELISA方法检测 30例膀胱癌患者、 2 5例良性血尿患者及 30例健康体检者尿脱落细胞的端粒酶活性。结果　 30例膀胱癌患者尿脱落细胞阳性例数 2 6例 (2 6 / 30 ) ,2 5例良性血尿患者及健康体检者均无端粒酶活性表达。结论　尿脱落细胞端粒酶活性的检测敏感性高、特异性好 。Objective Applying to a more sensitive and noninvasive assay for the diagnosis of bladder carcinoma, the authors tested the exfoliated cells from patients' voided urine for the presence of telomerase activity and evaluated its clinical significance.Methods TRAP-PCR-ELISA assay was used to determine the presence of telomerase activity in voided urine samples from patients with bladder carcinoma, hematuria of benign cause and from normal, health testees. Results Telomerase activity was detected in 26 of 30 tumor samples, while none of the hematuria samples and normal, healthy testees' samples. Conclusion Telomerase activity could be detected in most urine samples of bladder carcinoma patients and it is more sensitive and specific than other assays, therefore, would be a good diagnostic marker.

关 键 词：尿脱落细胞 端粒酶 膀胱癌 诊断 检测 

分 类 号：R737.14[医药卫生—肿瘤]