氟对大鼠破骨细胞基质金属蛋白酶-9的影响  

Effects of fluoride on matrix matalloproteinase-9 of osteoclast in rats

作　　者：孙波[1] 华坤[1] 李彤[2] 李广生[1] 

机构地区：[1]吉林大学生物工程研究所病理研究室,吉林长春130021 [2]吉林大学第二医院电诊科,吉林长春130051

出　　处：《中国地方病学杂志》2003年第3期193-194,共2页Chinese Jouranl of Endemiology

基　　金：国家自然科学基金重点资助项目 (3 973 0 3 90 )

摘　　要：目的　观察氟对大鼠破骨细胞分泌的基质金属蛋白酶 - 9(MMP- 9)的影响。方法　采用 westernblot技术观察染氟大鼠股骨干骺端 MMP- 9的表达 ;应用免疫组织化学方法观察氟对体外培养的破骨细胞MMP- 9的表达的影响。结果　染氟大鼠股骨干骺端 MMP- 9的蛋白表达增高 ;氟可使体外培养的破骨细胞MMP- 9的蛋白表达增高。结论　氟通过提高破骨细胞的 MMP- 9的表达而致破骨性骨吸收活性增强。Objective To investigate the effects of fluoride on matrix matalloproteinase-9(MMP-9) of osteoclasts in rats.Methods The MMP-9 expressions of femoral metaphysis in rats were investigated by western blot technique;using immunohistochemical stain, the MMP-9 expressions of cultivated osteoclasts were measured.Results The MMP-9 expressions of femoral metaphysis in fluoride groups elevated, and fluoride could make the expression of MMP-9 of cultivated osteoclasts increase.Conclusions Fluoride can increase osteoclastic bone resorption by elevating the MMP-9 expression of osteoclasts.

关 键 词：破骨细胞 氟化物 基质金属蛋白酶9 WESTERN blot技术 免疫组织化学 骨吸收活性 氟骨症 

分 类 号：R363[医药卫生—病理学]