选择性破坏胰腺A细胞的模型大鼠大脑皮层神经毡突触的电镜观察  

Ultrastructure Study of Synapses of Cerebral Cortex Nervous Felts in Model Rat with Selective Injured Islet A Cells

作　　者：张晓杰[1] 

机构地区：[1]齐齐哈尔医学院病理教研室,黑龙江齐齐哈尔161042

出　　处：《解剖科学进展》2003年第2期139-140,143,共3页Progress of Anatomical Sciences

基　　金：黑龙江省科学基金资助项目 (N995 5 )

摘　　要：目的　探讨选择性破坏胰腺A细胞的模型大鼠胆碱能神经元的变化。方法　选用硝酸钴和利血平选择性破坏大鼠胰腺A细胞后电镜观察大脑皮层神经毡胆碱能神经元突触的变化。结果　硝酸钴组模型大鼠脑突触内含胆碱能神经递质乙酰胆碱的清亮小泡比对照组明显减少 (P <0 .0 1) ,利血平组清亮小泡比对照组明显增多 (P <0 .0 5 )。结论　这种内源性乙酰胆碱的变化可能与胰腺A细胞损伤有关 ,提示胆碱能神经除受其上级中枢调节外 。Objective To study the relationship of the injury of islet A cell and cholinergic neurons in rat. Methods Co(NH 3) 2·6H 2O and Phenethyldiguanide were used to destroy inlet A cell selectively, and to observe the ultrastructure of synapse of cerebral cortex nervous felts. Results The number of synaptic vessicles containing acetylcholine decreased obviously in brain cortex nervous felts of cholinergic neurons in Co(NH 3) 2·6H 2O group (P<0.01), the number of synaptic vessicles increased obviously in Phenethyldiguanide group (P<0.05) compared with the control group respectively. Conclusion This change of endogenous acetylcholine may be related to the injury of islet A cell.

关 键 词：胰腺A细胞 动物模型 大脑皮层 神经毡突触 电镜 胆碱能神经元 硝酸钴 利血平 

分 类 号：R741[医药卫生—神经病学与精神病学]