苔藓植物DNA提取方法研究  

Study on extraction methods of bryophytes genomic DNA

作　　者：侯义龙[1,2] 曹同[1] 蔡丽娜[3] 孙志刚[3] 崔琳[3] 

机构地区：[1]中国科学院沈阳应用生态研究所 [2]大连大学生命科学工作室,辽宁大连116622 [3]大连大学生物工程学院

出　　处：《广西植物》2003年第5期425-428,435,共5页Guihaia

基　　金：国家自然科学基金 (30 1 70 0 76 ) ;上海市高等学校科学技术发展基金 (0 1 D0 3-2和 0 2 D2 -4 7)

摘　　要：提取高质量的 DNA是对苔藓植物遗传多样性进行研究的基础。该文以苔藓植物为试材 ,用 5种方法 ,即快速提取法、改良 CTAB法、CTAB法、SDS法及高盐法 (第一种为自行设计 ,第二种是对原有方法的改进 )对苔藓植物 DNA提取方法进行了比较研究。结果表明 ,快速提取法和改良 CTAB法是 2种适合于苔藓植物 DNA提取的方法。这 2种方法提取的 DNA浓度和纯度均比较高 ,凝胶电泳显示无明显降解现象 ,适宜作为 PCR扩增的模板 ,并成功地进行了 RAPD扩增。The extraction of high quality DNA was the basis of the study for genetic diversity of Bryophytes. The results of 5 kinds of method for extracting DNA were compared using Bryophytes species as materials. The results showed that the fast extraction method which was designed by our research group and improved CTAB method were 2 kinds of method suitable to the high quality DNA for Bryophytes. The purity of extracted DNA by the two methods was high and their fragment was clearly shown by agarose gel electrophoresis. DNA extracted by the two methods was ready to be used as templates for PCR amplification.

关 键 词：苔藓植物 DNA提取 RAPD扩增 提取方法 

分 类 号：Q949.35[生物学—植物学]