腺病毒介导的LacZ基因在B16小鼠黑色素瘤细胞中的表达  

Adenoviral-mediated LacZ gene transfer into the murine melanoma cell B16

作　　者：朱洁平[1] 吴云[1] 李慧[2] 曹广文[3] 徐容[1] 郑红[1] 潘卫[3] 邓松华[1] 

机构地区：[1]安徽医科大学病理生理学教研室,合肥230032 [2]上海复旦大学医学院,上海200032 [3]第二军医大学微生物教研室,上海200433

出　　处：《安徽医科大学学报》2003年第2期105-107,共3页Acta Universitatis Medicinalis Anhui

基　　金：安徽省教育厅自然科学基金资助研究项目 (编号 2 0 0 1Kj14 3)

摘　　要：目的　观察腺病毒介导的LacZ基因转移在B1 6细胞的转导效率。方法　腺病毒介导的LacZ基因转导B1 6细胞。结果　当MOI为 50～ 1 0 0 ,即可获得较高的转导效率 ,达 90 %± 1 %。结论　腺病毒介导的基因转移在B1 6细胞能获得较高的转导效率和目的基因的有效表达。提示腺病毒载体作为一种高效的基因转移载体 。Objective To observe the transduction efficiency of adenoviral-mediated LacZ gene transfer in the murine melanoma cells B16. Methods The B16 cells was transducted by recombinant adenovirus carrying LacZ gene in vitro. Results Transduction efficiency was as hi gh as 90%±1% at a multiply of infection (MOI) of 50～100.Conclusion Adenoviral-mediated gene transfer has a high transduction efficiency and stab le expression of gene of interest on the murine melanoma cells B16. The results suggest that adenovirus is a high-efficiency gene transfer vector and thus has bright prospect in the gene therapy of tumor.

关 键 词：腺病毒介导 LACZ基因 小鼠 黑色素瘤 B16细胞 基因治疗 

分 类 号：R739.5[医药卫生—肿瘤] R730.54[医药卫生—临床医学]