卵泡刺激素介导卵巢上皮性癌细胞增殖的初步研究  

Preliminary study on pathway of follicle-stimulating hormone on human epithelial ovarian cancer cell proliferation

作　　者：盛林[1] 刘东远[1] 沈铿[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院妇产科,100730

出　　处：《中华妇产科杂志》2003年第12期752-755,共4页Chinese Journal of Obstetrics and Gynecology

基　　金：国家自然科学基金资助项目 (3 990 0 15 7)

摘　　要：目的　探讨卵泡刺激素 (FSH)促进卵巢上皮性癌细胞增殖的作用途径 ,从分子水平上了解卵巢癌的发病机理。方法　卵巢上皮癌细胞系OVCAR3转染FSH受体表达质粒 ,获得FSH刺激敏感的细胞系OVCAR3 FSHR。以FSH刺激细胞 ,或与蛋白激酶C(PKC)的激活剂十四烷酰佛波醇乙酯 (TPA)和抑制剂他莫西芬 (TAM )共同刺激细胞 ,以四甲基偶氮唑蓝法检测细胞的增殖情况 ,逆转录 聚合酶链反应技术检测细胞PKC各亚型的mRNA表达 ;以蛋白印迹法观察PKCα及磷酸化PKCα的表达水平。结果　FSH可刺激OVCAR3 FSHR细胞增殖 1 9倍。FSH刺激后 ,PKCα的表达水平和活性分别增高 1 5倍和 1 9倍。TPA、TAM均能抑制FSH刺激OVCAR3 FSHR细胞增殖的6 0 %及 4 7% ,TPA或TAM与FSH共同刺激PKCα表达较FSH单纯刺激者明显降低。结论　FSH通过PKC的途径 ,可刺激卵巢上皮癌细胞增殖 ,在卵巢上皮癌的发展中起一定的作用。Objective There is only a limited direct indication that gonadotropins play a role in the genesis and development of epithelial ovarian cancer (EOC). Follicle-stimulating hormone (FSH) can enhance the growth of epithelial ovarian cancer cell in vitro.The research is to investigate the pathway of FSH action in epithelial ovarian cancer cell. Methods Epithelial ovarian cancer cell line OVCAR3 was transfected with FSH receptor cDNA expressing vector.The transfected cells that are sensitive highly to FSH stimulation were got,and named OVCAR3-FSHR.Adding FSH to the cells,or treating the cells with protein kinase C (PKC) activator tetradenocanoyl phorbol acetate (TPA),PKC inhibitor tamoxifen (TAM) in meantime,methyl thiazolyl tetrazolium (MTT)method was used to study the proliferation of cells. RT-polymerase chain reaction was used to identity the mRNA expression of various PKC subtypes. Westernblot was for detection of protein expression of PKCα and phosphorylated PKCα.Results FSH can promote proliferation of OVCAR3-FSHR(1.9 folds). There is some increase in PKCα by the FSH stimulation.The phosphorylated PKCα expression were enhanced significantly too. Both the amount and activity of PKCα were increased in response to FSH.TPA and TAM suppress FSH-stimulated cell growth (60% and 47%). Meanwhile expression level of PKCα was decreased with the co-treatment of TPA or TAM and FSH comparing with treatment with FSH only. Conclusions FSH promoted epithelial ovarian cancer cell proliferation through PKC pathway. It plays a role in the development of epithelial ovarian cancer.

关 键 词：卵泡刺激素 卵巢癌 癌细胞增殖 发病机理 蛋白激酶C 

分 类 号：R737.31[医药卫生—肿瘤] R730.2[医药卫生—临床医学]