胆管癌抗原肽对转染全长野生型p53的树突状细胞免疫功能的影响  

Effects of dendritic cells transfected with full length wildtype p53 and modified by bile duct cancer peptide on immune response

作　　者：孙华文[1] 杨清[2] 邹声泉[3] 

机构地区：[1]武汉大学人民医院普外科,430060 [2]武汉大学人民医院感染科,430060 [3]华中科技大学同济医学院附属同济医院普外科

出　　处：《中华实验外科杂志》2003年第7期626-628,共3页Chinese Journal of Experimental Surgery

摘　　要：目的　研究胆管癌抗原肽对转染全长野生型 p5 3的树突状细胞 (wtp5 3DC)免疫功能的影响。方法　将全长野生型 p5 3导入脂质体内并转染小鼠骨髓来源的DC ,然后用胆管癌抗原肽修饰wtp5 3DC ,检测这种树突状细胞的抗原提呈功能。结果　抗原肽修饰的wtp5 3DC和单纯DC这 4种上清 3种细胞因子含量明显增加为 :( 5 45 .2± 12 .1)ng/L ,( 5 11.1± 13 .3 )ng/L ,( 5 3 7.1±11.1)ng/L ;wtp5 3DC刺激小鼠脾脏T细胞增殖水平明显高于对照组 (P <0 .0 1) ;该细胞高表达B7 1、B7 2、MCH Ⅰ、MCH Ⅱ (P <0 .0 5 ) ;能够特异性地杀伤胆管癌细胞 ,杀伤率为 81.6%。结论全长野生型 p5 3基因转染与胆管癌抗原肽联合修饰树突状细胞能诱导小鼠细胞毒性T淋巴细胞的特异性。Objective To investigate the effects of dendritic cells transfected with full length wild type P53 and modified by bile duct cancer (BDC) peptide on immune response and whether the DC induce efficient and specific anti-tumor immunological response in vitro.Methods The wild type P53 was transducted to bone marrow-derived DCs (BMDC) with liposome,and the DCs were modified by bile duct cancer peptide.The functions of wt-P53DCs were detected.Results The contents of cytokines were increased in wtP53DCs modified by bile duct cancer peptide:(545.2±12.1) ng/L,(511.1±13.3) ng/L,(537.1±11.1) ng/L,P<0.05.The ability of wtp53 DC stimulating the proliferation of mouse splenic T cells was stronger than that in control or vector control group (P<0.01);The wtP53DCs highly express B7-1,B7-2,MCH-Ⅰ and MCH-Ⅱ (P<0.05),and specifically killed the cholangiocarcinoma cells with the killing rate being 81.6%.Conclusion The effects of dendritic cells transfected with wild type P53 and modified by bile duct cancer peptide on immune response have specific CTL killer ability in vitro.

关 键 词：胆管癌 抗原肽 野生型P53 树突状细胞 免疫功能 基因转染 

分 类 号：R735.8[医药卫生—肿瘤] R730.3[医药卫生—临床医学]