脑缺血后大鼠蝶腭神经节一氧化氮合酶表达的实验研究  

Expression of nitric oxide synthase in the sphenopalatine ganglion after cerebral ischemia in rats:an experimental study

作　　者：陆雪松[1] 姜亚军[1] 何家声[1] 李新胜[1] 

机构地区：[1]东南大学附属中大医院神经内科,江苏南京210009

出　　处：《现代医学》2003年第2期95-97,共3页Modern Medical Journal

摘　　要：目的　探讨一氧化氮合酶 (NOS)在大鼠蝶腭神经节内神经元中的表达与缺血再灌注脑损伤的关系。方法　 48只SD雄性大鼠 ,采用Pulisislli法制作闭塞大鼠四动脉的全脑缺血模型后随机分为实验组和假手术对照组 ,采用还原型尼克酰胺嘌呤二核苷酸脱氢酶 (NADPH d)组化法显示NOS阳性神经元 ,观察两组蝶腭神经节内NOS阳性神经元细胞数目的变化。结果　再灌注后NOS在蝶腭神经节内神经元表达水平增高 ,与假手术对照组相比 ,差异有极显著意义 (P <0 .0 1)。结论　NOS在大鼠缺血再灌注后蝶腭神经节内神经元中表达水平的增高 。Objective To explore the association between the expression of nitric oxide synthase(NOS) and cerebral ischemia reperfusion in rats.Methods Pulisislli 4-vessel occlusion method was used to establish cerebral ischemia model in 48 male SD rats.Then,the rats were randomly divided into two groups:the study group and sham operative control group.NADPH-d histochemistry was used to detect the expression of NOS in sphenopalatine ganglion.Result The expression of NOS in study group was significantly higher than that in control group(P<0.01).Conclusion The increased expression of NOS in the sphenopalatine ganglion after cerebral ischemia reperfusion in rats may be a protective reaction during cerebral ischemia.

关 键 词：脑缺血 大鼠 蝶腭神经节 一氧化氮合酶 实验 再灌注脑损伤 

分 类 号：R743.31[医药卫生—神经病学与精神病学] R-33[医药卫生—临床医学]