E-钙粘附素和基质金属蛋白酶的表达与胃癌侵袭、转移的关系  

The relation of E-cadherin and MMP-2 expression to the invasion and metastatic of gastric cancer

作　　者：汪泳[1] 刘昕[1] 朱任之[1] 杨树琛 张建刚[1] 钱震[3] 

机构地区：[1]兰州医学院病理生理学教研室,甘肃兰州730000 [2]甘肃省定西地区医院,甘肃定西743000 [3]兰州军区总医院,甘肃兰州730000

出　　处：《中国现代医学杂志》2004年第1期69-71,共3页China Journal of Modern Medicine

摘　　要：目的　探讨E -钙粘附素 (E -cadherin)和基质金属蛋白酶 - 2 (matrixmetalloporoteirnase - 2 ,MMP- 2 )在胃癌组织中的表达与胃癌浸润转移的相关性。方法　收集住院胃癌手术病例 83例 ,同时记录相关临床资料。 2 0例胃良性病变手术病例。采用S -P免疫组化染色 ,检测组织的E -cadherin和MMP - 2的表达情况 ,同时行HE染色对胃癌进行组织学分型。结果　正常胃黏膜E -cadherin呈强阳性表达 ,胃癌组织中E-cadherin表达率明显减低 ,阳性表达率为 16 .9% (14 / 83) ,E -cadherin表达减低与胃癌细胞分化差、浸润深度、弥漫性生长、淋巴结转移密切相关 (P <0 .0 5 ) ;而与TNM分期及肿瘤大小未见明显相关性。MMP - 2在正常胃黏膜中呈阴性表达 ,在胃癌组织中表达呈阳性 ,阳性率为 78.3% (6 5 / 83) ,MMP - 2表达强度与胃癌分化程度、淋巴结车瞄、浸润深度、弥漫性生长、TNM分期显著相关 (P <0 .0 5 ) ;而与肿瘤大小未见明显相关性。结论　E -cadherin的表达下调和MMP - 2表达上调将促进胃癌浸润和转移 ,二者在癌组织中表达的高低是胃癌细胞重要的恶性生物学特征 ,有助于对胃癌转移及病人预后的判断。Objective:To investigate the expression of E-cadherin and Matrix metalloproteinase-2(MMP2) in human gastric cancer and their relationship with tumor cell invasion and metastasis.Methods:The collection of expession of E-cadherin and Matrix metalloproteinase-2 of gastric mucosa were detected in 20 cases of health control group, 83 cases of gastric cancer by using streptavidin-peroxidase immunohistochemistry.Results:All the nomal gastric mucosa abundantly expressed E-cadherin,but did not express MMP 2. The exprestion of E-cadherin and MMP 2 were 16.9% and 78.3% respectively in the gastric cancer group. The related factors with E-cadherin expression reduction were lower differentiation, depth of infitration, diffusive growth and lymphaden metastasis of gastric cancer cell.The related factors with MMP2 expression were the differentiation degree, lymphaden metastasis, depth of infiltration, diffusive growth and TNM stadium of gastric cancer cell. The non-related factors with E-cadherin expression reduction were the TNM stadium and the size of gastric cancer. The non-related factor with MMP2 expression was the size of gastric cancer.Conclusions:The declining and ascendence expressions of E-cadherin and MMP2 are significantly related with the infiltration and transferring of gastric cancer cell. The changes of the two expressions are the important biological characteristics of gastric cancer cell and can help the judgment of the gastric cancer transferring and the prognosis.

关 键 词：胃肿瘤 E-钙粘附素 基质金属蛋白酶-2 免疫组化 

分 类 号：R363.21[医药卫生—病理学]