Akt信号通路在HD02抗慢性前脑缺血大鼠神经细胞凋亡中的作用  

Effect of Akt signal pathway on neuron apoptosis in forebrain anti-chronic ischemia rats treated with HD02

作　　者：高唱[1] 吴伟康[1] 

机构地区：[1]中山大学中山医学院中西医结合研究所,广东省广州市500080

出　　处：《中国临床康复》2004年第1期56-57,共2页Chinese Journal of Clinical Rehabilitation

基　　金：国家自然科学基金资助项目(30271652)~~

摘　　要：目的:观察Akt信号通路在HD02抗大鼠慢性前脑缺血过程中神经细胞凋亡中的作用。方法:采用流式细胞仪和Westernblot方法检测细胞凋亡率和Akt信号通路犤总Akt,磷酸化Akt(Ser473),Akt(Thr308),FKHR(Ser256),GSK-3β(Ser9)和PTEN犦变化。结果:与正常对照组犤(1.40±0.21)%犦比较,造模后15和30d慢性前脑缺血大鼠细胞凋亡率犤(15.30±0.74),(12.72±3.45)%犦显著增加(t=7.56,7.02,P<0.01);磷酸化Akt(Ser473)和Akt(Thr308)表达水平显著降低(t=3.51,3.76,P<0.01);FKHR(Ser256)、GSK-3β(Ser9)和PTEN表达明显增加。HD02可明显降低慢性前脑缺血大鼠细胞凋亡率犤HD0215d:(11.40±0.75)%,HD0230d:(5.40±1.38)%犦。与模型组比较,给药后15和30d,HD02可显著上调磷酸化Akt(Ser473)和Akt(Thr308)表达水平(t=3.94,4.18,P<0.01);明显抑制FKHR(Ser256),GSK-3β(Ser9)和PTEN表达。结论:HD02能抑制慢性前脑缺血大鼠神经细胞凋亡,这一作用与Akt信号通路有关。AIM:To explore the effect of Akt signal pathway on neural apoptosis in forebrain anti chronic ischemia rats treated with HD02. METHODS: Flow cytometry and Western Blot method were used to detect the changes of cellular apoptosis rate and Akt signal pathway[total Akt, phosphorylase Akt(Ser473),Akt(Thr308), FKHR(Ser256),GSK 3β(Ser9) and PTEN]. RESULTS: Compared with the normal control group [(1.40±0.21)%],cellular apoptosis rate was significantly increased in the chronic forebrain ischemia rats 15 and 30 d[(15.30±0.74)%,(12.72±3.45)%]after model making(t=7.56,7.02,P< 0.01).The expressive level of phosphorylase Akt(Ser473) and Akt(Thr308) were significantly decreased(t=3.51,3.76,P< 0.01) and the level of FKHR(ser256),GSK 3β(Ser9) and PTEN were significantly increased.HD02 could reduce the apoptosis rate of chronic forebrain ischemia rats[HD02 15 d:(11.40±0.75)%,HD02 30 d: (5.40±1.38)%].15 d and 30 d after medication,HD02 increased the expressive level of phosphorylase Akt(Ser473) and Akt(Thr308) significantly(t=3.94,4.18,P< 0.01),and reduced those of FKHR(ser256),GSK 3β(Ser9) and PTEN significantly compared with the model group. CONCLUSION: HD02 could effectively inhibit the neuronal apoptosis in forebrain ischemia rats. Akt pathway is involved in the neuron protective of HD02.

关 键 词：AKT信号通路 HD02 慢性前脑缺血 大鼠 神经细胞 细胞凋亡 中药 

分 类 号：R743.3[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]