大鼠中度脑损伤后行为和认知功能障碍的测定  

Determinations of the behavioral and cognitive dysfunction after moderate traumatic brain injury in rats

作　　者：陈世文[1] 马霞[2] 陈建文 刘子生[1] 

机构地区：[1]解放军济南军区总医院神经外科,山东省济南市250031 [2]山东轻工业学院食品工程系,山东省济南市250100 [3]日照市卫生防疫站生化检疫科,山东省日照市276800

出　　处：《中国临床康复》2004年第1期48-49,共2页Chinese Journal of Clinical Rehabilitation

摘　　要：目的:探讨大鼠中度脑损伤后不同行为功能和记忆功能测定方法的效能。方法:将成年健康雄性SD大鼠20只,单纯随机分成两组,手术准备后致伤,测定中度液压脑损伤后大鼠不同时期的行走、平衡、爬坡和记忆功能评分。结果:实验组大鼠伤后第1天行走试验时间明显延长,为(20.95±3.36)s,至伤后第7天仍未恢复正常,为(5.12±0.71)s,与对照组(3.54±0.82)s比较,t=4.6064,P<0.01。平衡试验:实验组伤后第1天评分为4.20±1.00,至伤后第6天恢复正常,评分为1.13±0.35,与对照组1.00±0.00比较,t=1.1746,P>0.05。用记忆功能测定箱测试时,实验组大鼠至第13天记忆功能仍未恢复,评分为5.3±1.8,与同一天对照组7.1±1.2比较,t=2.4437,P<0.05。实验组大鼠伤后爬坡能力至第4天即恢复至(51.7±3.5)°,与对照组(53.6±3.8)°比较,差异并无显著性意义(t=1.1630,P>0.05)。结论:伤后大鼠行走、平衡和记忆功能障碍明显,且持续到伤后较长时间,而爬坡能力障碍较轻。AIM:To explore the efficacy of determining methods of different behavioral and cognitive function after moderate traumatic brain injury(TBI) in rats. METHODS:Twenty healthy male SD rats were randomly divided into 2 groups, prepared for operation, then the rats were injured. The scores of beam walking, beam balancing, slope climbing test and cognitive function were determined after moderate fluid pressure TBI. RESULTS:The duration of beam walking at the 1st day after injury in the study group [(20.95±3.36)s] prolonged obviously, and hadn't turned to be normal until the 7th day[(5.12±0.71) s], as compared with the control group[(3.54±0.82)s] (t=4.606 4,P >0.01). The score of beam balancing at the 1st day after injury in the study group was(4.20±1.00), and turned to be normal at the 6th day(1.13±0.35), while that in the control group was (1.00±0.00) (t=1.174 6,P >0.05). The cognitive function in the study group hadn't turned to be normal until the 13th day after injury (5.3±1.8), and that in the control group was(7.1±1.2)(t=2.443 7,P< 0.05). The slope climbing ability in the study group recovered just at the 4th day after injury(51.7±3.5), it was not significantly different from that in the control group(53.6±3.8)(t=1.163 0,P >0.05). CONCLUSION:The dysfunctions in beam walking, beam balancing and cognition are significant in rats after traumatic brain injury, and can last for a long time, while that in slope climbing ability is light.

关 键 词：大鼠 脑损伤 认知功能障碍 手术 神经功能 

分 类 号：R651.15[医药卫生—外科学]