海洋真菌岩藻多糖酶的固态发酵条件研究  

SOLID STATE FERMENTATION OF FUCOIDANASE OF MARINE FUNGUS DENDRYPHIELLA ARENARIA TM94

作　　者：吴茜茜[1] 吴克[1] 蔡敬民[1] 秦松[2] 张洁[1] 严小军[2] 潘仁瑞[1] MEINERS M. 

机构地区：[1]合肥学院,合肥230022 [2]中国科学院海洋研究所,青岛266071 [3]University of Applied Sciences ldenburg

出　　处：《菌物系统》2003年第3期441-444,共4页Mycosystema

基　　金：中国科学院实验海洋生物学重点实验室研究报告第304号;安徽省自然科学基金资助项目(03043104)

摘　　要：本文对海洋真菌Dendryphiella arenaria(TM94)岩藻多糖酶的固态发酵条件进行了研究,主要内容包括碳源、氮源、添加物、起始pH、接种量及温度等。固态发酵最佳培养基组成:麸皮7.5g,葡萄糖0.5g,海带粉0.6g,NaNO3为4g/L;最佳培养条件为培养温度28℃,起始pH6,接种量3ml (孢子浓度为106个/ml)。在28℃培养24h,酶活力可达35.5 IU/g干培养基,比活力为1.39 IU/mg蛋白质。对岩藻多糖酶酶学性质也进行了研究。Solid state fermentation conditions of Dendryphiella arenaria TM94 fucoidanase were studied. The optimal medium contained wheat bran 7.5g, glucose 0.5g, kelp 0.6g and NaNO3 4g/L. The optimal initial pH and inoculum were 6.0 and 3ml(spore concentration 106/ml) respectively. Under the condition of 28℃, fucoidanase activity reached 35.5 IU/g dry medium after 24h, and its specific activity was as high as 1.39 Iu/mg. The fucoidanase properties of Dendryphiella arenaria were also studied.

关 键 词：岩藻多糖酶 固态发酵 酶学性质 

分 类 号：TQ925[轻工技术与工程—发酵工程]