杜氏盐藻核基质的制备  

Preparation of nuclear matrix of Dunaliella salina

作　　者：王天云[1] 柴玉荣[1] 侯卫红[1] 谢华[1] 赵青赞[1] 王宁[1] 薛乐勋[1] 

机构地区：[1]郑州大学细胞生物学研究室

出　　处：《郑州大学学报（医学版）》2004年第1期39-41,共3页Journal of Zhengzhou University(Medical Sciences)

基　　金：国家自然科学基金资助项目 3 0 2 70 0 3 1;国家高技术研究发展 ( 863 )计划 2 0 0 2AA62 80 5 0

摘　　要：目的 :制备盐藻核基质 ,进而分离核基质结合区 (matrixattachmentregions)。方法 :采用体积分数 0 .5 %TritonX - 1 0 0破碎细胞 ,体积分数 1 5 %Percoll分离盐藻细胞核 ,二碘水杨酸锂 (lithiumdiiodosalicylate,LIS)抽提核蛋白 ,SDS -PAGE电泳分析蛋白质成分。结果 :分离出了高纯度的盐藻细胞核 ,电泳分析表明绝大部分核蛋白已被去除。结论 :体积分数 0 .5 %TritonX - 1 0 0能有效破碎盐藻细胞 ,体积分数 1 5 %Percoll可分离高纯度的盐藻细胞核 ,2Aim: Through preparing nuclear matrix of Dunaliella salina, in order to isolate the matrix attachment regions further.Methods: The D.salina cells were disrupted with 0.5%TritonX-100 and purified by centrifugation using 15% Percoll.The nuclear proteins were removed using 25 mmol/L lithium diiodosalicylate and the component of protein was analyzed by SDS-PAGE. Results: The pure nuclei of D.salina were isolated, and most protein component of nuclear protein was removed. Conclusion: The matrix of D.salina may be prepared through 25mmol/L LIS extracting nuclear protein.

关 键 词：盐藻 核基质 核基质结合区 SDS—PAGE 

分 类 号：Q781[生物学—分子生物学]