抗胸腺细胞血清性肾炎模型大鼠肾小球中C5b-9的沉积及NO、TNFα含量分析  

Analysis of glomerular complement C5b-9 deposits and synthesis of NO, TNFα in the model of the rats with anti-thymocyte serum nephritis

作　　者：王迎伟[1] 徐静华[1] 汤仁仙[2] 高丰光[2] 

机构地区：[1]南京医科大学免疫学教研室,江苏南京210029 [2]徐州医学院免疫学教研室,江苏徐州221002

出　　处：《中国病理生理杂志》2004年第1期54-59,共6页Chinese Journal of Pathophysiology

基　　金：江苏省"3 3 3工程"专项基金资助项目 (No.2 0 0 78);江苏省自然科学基金资助课题 (No .BK97154)

摘　　要：目的 :探讨抗胸腺细胞血清性肾炎 (ATSN)大鼠肾小球内C5b -9复合物的沉积与某些炎症介质和细胞因子如 :一氧化氮 (NO)和肿瘤坏死因子α(TNFα)的含量情况。方法 :大鼠一次性静脉注射抗胸腺细胞抗血清(ATS)建立ATSN模型 ,定期对ATSN大鼠肾小球中的补体C5b -9复合物进行免疫组化染色定位、显微图像扫描半定量分析 ;并对有C5b -9包绕的肾小球系膜细胞 (MC)进行计数。测定ATSN大鼠肾中诱生型NO合酶 (iNOS)mRNA的表达、尿液中NO的代谢产物 (NO-2 /NO-3 )及TNFα的排泄量。结果 :ATSN模型大鼠肾小球MC先溶解坏死后继发增生 ,病变早期 (溶解时相 )补体C5b -9复合物主要定位于肾小球系膜区及MC表面 ;随着病程的进展 ,被C5b -9包绕的MC逐渐减少 ,病程初期ATSN大鼠肾小球MC有明显的iNOSmRNA表达 ,尿液中NO-2 /NO-3 和TNFα的排泄量也明显增加。在ATSN病变的增生阶段 (一般 7d后 ) ,上述指标的变化逐渐趋缓。结论 :ATSN模型大鼠肾小球中MC逐渐溶解与补体C5bAIM: To explore the localization and semi-quantification o f the glo merular complement C5b-9 complexes and synthesis of some inflammatory mediators or cytokines such as nitric oxide (NO) and tumor necrosis factor α(TNFα) in the ra ts with anti-thymocyte serum nephritis(ATSN). METHODS: The animal mo del of rat AT SN was reproduced by a single intravenous injection of anti-thymocyte serum (ATS ). Then, the deposits of glomerular C5b-9 complexes were localized and quantifie d by immunohistochemical staining and microscopic image scanning separately. And the glomerular mesangial cells (MC) surrounded by C5b-9 complexes were counted under microscope. In addition, the expression of glomerular MC inducible NO synt hase(iNOS) mRNA and excretion of urinary NO metabolite ( NO - 2/NO - 3 ) and TNF α in the rats with ATSN were detected. RESULTS: The MC in t h e rats with ATSN emerged necrosis followed by a rapid proliferation. In the early time of MC injury, the C5b-9 complexes were mainly seen in glomerular mesangium and MC sur fac e. But with the progression of ATSN, the MC enclosed by C5b-9 appeared gr adual decrease. Moreover, the expression of MC iNOS mRNA in early stage of ATSN obviously increased and the excretion of urinary NO - 2/NO - 3 and TNF α also significantly increased. However, the changes of parameters mentioned abov e in ATSN proliferative stage (after 7 days) alleviated gradually. CONCLUSION: The second ary lysis of MC has relation to the deposition of C5b-9 complexes and synthesis and release of NO and TNF α in rats with ATSN.

关 键 词：抗胸腺细胞血清性肾炎 补体膜攻击复合物 一氧化氮 肿瘤坏死因子Α 大鼠 肾小球 C5B-9 NO TNFΑ 

分 类 号：R692.3[医药卫生—泌尿科学]