MDM2和P53蛋白表达与原发性肝细胞肝癌发生的关系  

Relationship between protein expression of MDM2 and P53 and hepatocar cinogenesis

作　　者：潘运龙[1] 覃莉[2] 蒋光愉[3] 严林[1] 王华曦[1] 

机构地区：[1]暨南大学医学院第一附属医院外科,广东广州510632 [2]暨南大学医学院组织胚胎学教研室,广东广州510632 [3]暨南大学医学院第一附属医院病理科,广东广州510632

出　　处：《中国病理生理杂志》2004年第1期72-75,共4页Chinese Journal of Pathophysiology

基　　金：广东省自然科学基金资助项目 (No .0 10 3 67);广东省卫生厅基金资助项目 (No .A19992 94)

摘　　要：目的 :研究MDM2和突变型P53蛋白表达与原发性肝细胞肝癌 (HCC)发生的关系。方法 :用免疫组织化学SP法 ,检测 55例HCC癌组织、2 3例癌旁组织、10例正常肝组织MDM2和突变型P53蛋白表达情况。结果 :55例HCC中MDM2蛋白阳性表达 17例 (3 0 9% ) ,突变型P53蛋白阳性表达 2 3例 (41 8% ) ,二者均阳性表达 11例(2 0 % ) ,MDM2和突变型P53蛋白表达有相关性 (r=0 3 10 ,P <0 0 5)。 2 3例癌旁组织MDM2蛋白阳性 1例 ,突变型P53蛋白阳性表达 2例 ,肝癌组织和癌旁组织MDM2和突变型P53蛋白表达有差异 (P <0 0 5)。 10例正常肝组织无MDM2和突变型P53蛋白表达。结论 :肝细胞癌组织有MDM2和突变型P53过量表达 ,MDM2蛋白和AIM: To study the expression of MDM2 and muta nt-type P53 proteins in primary hepatocellular carcinoma(HCC). METHODS: Using immunohistochemical staining method(SP),the expression of mutant-t ype P53 and MDM2 proteins was examined in 55 cases of HCC,23 cases of correspond ing paracancerous tissues and 10 cases of normal hepatic tissues. RESULT S: The frequencies of MDM2 and P53 positive expression in HCC were 17/55 (30 9%) and 23/55 (41 8%), respectively. There was both positive expression of MDM2 and mutant-type P53 in 11 cases (20%) with HCC. Expression of MDM2 showed a significantly positive correlation with expression of mutant-type P53 (r=0 310,P<0 05). The frequencies of MDM2 and P53 positive expression in correspo nding paracancerous tissues cells were 1/23 (4 3%) and 2/23(8 6%), respectivel y. Otherwise, there was a significant statistical difference in MDM2 and mutant- type P53 protein expression between HCC and paracancerous tissues(P<0 05). There was not positive expression of MDM2 and mutant-type P53 in normal hepatic tissue. CONCLUSION: There is MDM2 and mutant-type P53 protein o verexpression in HCC. The p53 gene inactivation resulting fr om MDM2 protein overexpression and/or p53 gene mutation may play an importan t role in hepatocarcinogenesis.

关 键 词：原发性肝细胞肝癌 MDM2蛋白质 P53蛋白质 基因表达 免疫组织化学 发病机制 

分 类 号：R735.7[医药卫生—肿瘤] R730.2[医药卫生—临床医学]