褪黑素对异丙肾上腺素诱导的大鼠海马tau蛋白过度磷酸化和空间记忆损伤的保护作用(英文)  

Protection of melatonin on isoproterenol-induced hyperphosphorylation of tau and spatial memory impairment in rats

作　　者：凌智群[1] 王小川[1] 张少华[1] 张业平[1] 王建枝[1] 

机构地区：[1]华中科技大学同济医学院病理生理学系,湖北武汉430030

出　　处：《中国病理生理杂志》2004年第1期6-11,共6页Chinese Journal of Pathophysiology

基　　金：bytheFoundationoftheNationalNaturalScienceFoundationofChina (No .3 992 50 12 ;3 0 10 0 0 57;3 0 170 2 2 1)andthegrantnumberedG19990 540 0 7

摘　　要：目的 :在大鼠整体研究褪黑素对异丙肾上腺素 (IP)诱导的tau过度磷酸化和动物保留记忆损伤的保护作用。方法 :大鼠海马双侧注射IP或同时注射褪黑素 (0 1μg ,1μg) ,利用免疫组化、免疫印迹和Morris水迷宫等指标 ,观察褪黑素的效果。结果 :0 1μg和 1μg剂量的褪黑素明显降低IP引起的海马骨架蛋白TauSer3 96/ 40 4位点的过度磷酸化 ;1μg褪黑素可明显改善IP引起的动物保留记忆损伤。 结论AIM: To study the in vivo effect of melatonin (MEL) on Alzheimer-like tau hyperphosphorylation and spatial memory retention impairment induced by iso proterenol (IP). METHODS: Simultaneous injection of 2 μL IP( 10 mmol/L) or IP plus MEL(0 1 μg,1 μg)into rat hippocampus bilaterally w i th stereotexin; The phosphorylation of tau was analyzed by immunohistochemistry and Western blot and the memory retention was tested by Morris Water Maze. RESULTS: Injection of IP induced hyperphosphorylation of tau at Tau-1 and PHF-1 epitopes and increased latency o f rats to find the hidden platform in the Morris water maze. MEL significantly r estored the IP-induced impairment in spatial memory(P<0 05)and tau hyper p hosphorylation determined by both immunocytochemistry and Western blot. CONCLUSION: MEL inhibits partially the IP-induced pathological process es in tau hyperphosphorylation and spatial memory impairment in rat brain.

关 键 词：褪黑激素 TAU蛋白质类 异丙肾上腺素 大鼠 海马 过度磷酸化 空间记忆损伤 保护作用 

分 类 号：R363[医药卫生—病理学]