原位杂交定位检测神经抑制再生基因Nogo-A mRNA体外培养的视神经少突胶质细胞的表达  

Detection and localization of Nogo-A mRNA expression in cultured oligodendrocytes isolated from optic nerve using in situ hybridization

作　　者：彭锡嘉[1] 刘少章[1] 叶剑[1] 袁容娣[1] 

机构地区：[1]第三军医大学大坪医院眼科

出　　处：《重庆医学》2004年第1期46-48,共3页Chongqing medicine

基　　金：国家自然科学基金资助项目 (30 2 70 4 58);重庆市科委应用基础研究资助项目 (41A1 1 52C);全军十五医药卫生科研基金资助项目(0 1MA1 75)。

摘　　要：目的　观察神经生长抑制因子Nogo -A在体外培养的视神经少突胶质细胞的表达。 方法　用地高辛标记的寡核苷酸探针少突胶质细胞进行原位杂交 ,检测Nogo AmRNA表达。结果　原位杂交显示少突胶质细胞胞质中均呈现浓淡不一的淡黄色着色 ,细胞核不着色。结论　原位杂交结果从基因水平证明 ,视神经少突胶质细胞能合成可以合成神经生长抑制因子Nogo A。Objective To determine whether Nogo A mRNA a kind of neurite growth inhibitors expresses in cultured oligodendrocytes in vitro.Method The presence of Nogo A mRNA was investigated by in situ hybridization(ISH ) with digoxin labeled oligonucleotide probes in cultured oligodendrocytes.Results Nogo A mRNA positive cells showed light to dark yellow staining in oligodendrocytes cytoplasm.Conclusion The presence of mRNA encoding for Nogo A (NI 250) effectively detectedby ISH in cultured oligodendrocytes isolated from newborn rat optic nerve.

关 键 词：视神经 少突胶质细胞 细胞培养 原位杂交 NOGO 

分 类 号：R774.6[医药卫生—眼科]