人卵泡抑素基因全长cDNA的克隆  

Cloning of full length cDNA of human follistatin gene

作　　者：尤汉宁[1] 李定国[1] 黄新[1] 陈颖伟[1] 张晶[1] 刘清华[1] 徐芹芳[1] 

机构地区：[1]上海第二医科大学新华医院消化内科,上海200092

出　　处：《上海第二医科大学学报》2004年第1期18-20,共3页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：国家自然科学基金资助项目(30170411)

摘　　要：目的 克隆人卵泡抑素(FS)全长cDNA基因。方法 采用逆转录多聚酶链反应(RT-PCR)从人肝组织中扩增FS cDNA,构建PMD18-T载体,采用BamH Ⅰ、HindⅢ双酶切以及DNA测序进行鉴定。结果 RT-PCR扩增长度为968 bp的基因片段,BamH Ⅰ、HindⅢ双酶切结果显示重组T载体中含有目的基因片段,测序结果显示编码区无基因突变。结论 人FS全长cDNA基因克隆成功,并构建了重组PMD18-T载体。Objective To clone full length cDNA of human follistatin gene. Methods The full length human follistatin cDNA gene was amplified successfully from human liver tissues using RT-PCR technique, and then cloned into PMD18-T vector, which was identified and confirmed by restriction endonuclease mapping using BamH Ⅰ and HindⅢ as well as DNA sequence analysis. Results The human follistatin DNA containing 968 bp was amplified using RT-PCR, restriction endonuclease mapping using BamH Ⅰ and HindⅢ showed that targeting DNA was inserted into recombinant PMD18-T vector, and there was no mutation in the coding area through DNA sequencing. Conclusion The full length human follistatin cDNA gene was successfully cloned and recombinant PMD18-T vector was constructed.

关 键 词：卵泡抑素 基因 CDNA 克隆 T载体 

分 类 号：R346[医药卫生—基础医学]