PDGF-BB引起系膜细胞内游离钙浓度变化的机制探讨  

Study of mechanism of intracellular calcium concentration elevation induced by PDGF-BB in glomerular mesangial cells

作　　者：张翀[1] 温莉萍[1] 朱汉威[1] 蒋更如[1] 

机构地区：[1]上海第二医科大学新华医院肾脏内科,上海200092

出　　处：《上海第二医科大学学报》2004年第1期21-24,共4页Acta Universitatis Medicinalis Secondae Shanghai

基　　金：上海市教委青年基金资助项目(98QN63)

摘　　要：目的 研究血小板衍生生长因子BB(PDGF-BB)引起的肾小球系膜细胞内游离钙浓度变化及其离子流动机制。方法以体外培养的系膜细胞为研究对象,激光共聚焦显微术结合Fura-3染色法动态测定细胞内游离钙浓度。结果 PDGF-BB可浓度依赖性地引起肾小球系膜细胞内游离钙浓度的升高,细胞内钙库释放抑制剂TMB-8、无钙缓冲液和钙通道阻滞剂硝苯地平均可抑制PDGF-BB引起的细胞内钙浓度变化,但其抑制作用发挥的时间和程度均有显著不同。结论 PDGF-BB引起肾小球系膜细胞内游离钙浓度的升高在初期是通过细胞内钙释放与细胞外钙内流共同参与,而在其后的维持升高阶段主要是通过细胞外钙内流,特别是通过电压依赖式钙通道内流引起。Objective To investigate the intracellular calcium concentration alteration induced by platelet-derived growth factor BB(PDGF-BB) and the mechanism of ion flux in cultured glomerular mesangial cells. Methods Rat mesangial cells were cultured. Intracellular calcium concentrations were measured by confocal laser scanning microscopy and Fura-3 fluorescence dyeing techniques. Results PDGF-BB significantly increased intracellular calcium concentrations in a dose-dependent manner. Intracellular calcium release blocker (TMB-8) , Ca2+ -free bath, and calcium channel blocker (nifedipine) all inhibited the elevation of intracellular calcium concentrations induced by PDGF-BB , but the temporal pattern and magnitude of the inhibitory effect were also significantly different. Conclusion The initial phase of PDGF-BB-induced [Ca2+] elevation was due to both the release of intracellular calcium stores and extracellular calcium entry, but in the latter phase, extracellular calcium entry, especially through voltage-operated Ca2+ channel, is the main cause.

关 键 词：PDGF-BB 系膜细胞 游离钙 浓度 机制 肾小球炎症 

分 类 号：R363[医药卫生—病理学]