转mtlD/gutD双价基因稻米对小鼠的毒性试验  

A Toxicity Test of Transgenic Rice with a mtlD/gutD Gene on Mice

作　　者：王慧中[1] 应奇才[2] 钟国庆[3] 黄大年[4] 钱前[4] 张红生[1] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,南京210095 [2]杭州师范学院生化与分子生物学重点实验 [3]杭州师范学院生化与分子生物学重点实验室,杭州310036 [4]中国水稻研究所,杭州310006

出　　处：《中国粮油学报》2003年第6期16-19,共4页Journal of the Chinese Cereals and Oils Association

基　　金：浙江省自然科学基金项目 (30 2 0 61 );中国水稻科学发展基金(0 0 0 32 39);浙江省科技计划项目 (0 1 1 1 0 2 1 86)

摘　　要：对转mtlD/gutD双价基因稻米进行了小鼠急性亚急性毒性实验、致突变试验和 30天喂养试验。结果表明 :小鼠经口LD50 >30g/kg体重 ,无致突变作用 ,30天喂养试验 18g/kg、36g/kg、5 4g/kg体重各剂量组小鼠发育、增重、食物利用率、血常规、脏体比及病理组织学观察等各项指标与基础对照组比均无显著性差异 ,无作用剂量为 5 4g/kg体重。表明转mtlD/gutD双价基因稻米对小鼠没有毒性 。Acute toxicity experiments, mutation experiments and 30-day feeding test were conducted on mice with transgenic rice barring a mtlD/gutD gene. The results showed that oral LD 50 for mice was higher than 30g/kg and no mutation was found. The mice of 18g/kg,36g/kg,54g/kg-dosage groups had good development in 30-day feeding test. Compared with those of negative control group, the weight, food utilization rate, routine blood test, rate of organ to body(weight to weight),and patho-histological observations had no obvious difference. None effect dosage was 54g/kg.So the conclusion was that the transgenic rice with a mtlD/gutD gene was safe to take.

关 键 词：转基因稻米 急性毒性实验 致突变试验 动物实验 安全性 

分 类 号：TS201.6[轻工技术与工程—食品科学] Q789[轻工技术与工程—食品科学与工程]