电刺激小脑顶核促脑缺血后毛细血管新生的实验研究  

Role of fastigial nuclei stimulation on angiogenesis in rats with local cerebral ischemia

作　　者：石正洪[1] 董为伟[2] 

机构地区：[1]兰州医学院第二附属医院神经内科 [2]重庆医科大学神经病学研究所,重庆400016

出　　处：《中风与神经疾病杂志》2003年第3期250-252,共3页Journal of Apoplexy and Nervous Diseases

基　　金：国家自然科学基金资助项目(No.39370 2 6 1)

摘　　要：目的　探讨电刺激小脑顶核 (FNS)对局部脑缺血后毛细血管新生的影响。方法　以线栓法制成大鼠右侧大脑中动脉梗死 (MCAO)模型 ,大鼠随机分为假手术对照组、MCAO组、FNS(MCAO +FNS)干预组。以免疫组织化学法检测内皮细胞阳性表达及毛细血管记数。结果　大脑中动脉梗死后 ,缺血区神经元变性、坏死 ,内皮细胞在半暗带有少量表达 ,毛细血管数较对照组增加 ,经FNS干预后 ,内皮细胞大量表达 ,毛细血管数目明显增加 ,有统计学差异。结论　FNS可通过促内皮细胞增殖 ,从而保护脑毛细血管内皮细胞 ,促进毛细血管新生。Objective To investigate the role of fastigial nuclei stimulation(FNS)on angiogenesis in rats with local cerebral ischemia. Methods Mature Wistar rats were randomly divided into three groups:sham operated control group,MCAO group (right middle cerebral artery occlusion) and MCAO+FNS group. The expressions of endothelial cells(EC)and capillary density(CD)were assayed by using immunohistochemical staining and image analysis. Results There was EC expression in the areas of cerebral ischemia. After treated by fastigial nuclei stimulation,the numbers of EC and CD increased remarkablely. Conclusion Angiogenesis after local cerebral ischemia induced by fastigial nuclei stimulation might result from increasing the expression of endothelial cell.

关 键 词：电刺激 小脑顶核 脑缺血 毛细血管新生 实验研究 免疫组织化学法 大脑中动脉梗死 

分 类 号：R743.3[医药卫生—神经病学与精神病学]