^(90)钇标记单克隆抗体LC1对荷肺癌裸鼠局部放射免疫治疗  

Topical Radioimmunotherapy of Lung Cancer in Nude Mice Using Monoclonal Antibody LC1 Labeled with ^(90)Y

作　　者：葛棣[1] 曾亮[1] 葛锡锐[2] 林斯骏 钟高仁[3] 朱建华[3] 

机构地区：[1]复旦大学附属中山医院胸外科 [2]中国科学院上海细胞生物学研究所 [3]复旦大学药学院放射药学教研室,上海200032

出　　处：《复旦学报（医学版）》2003年第2期160-163,共4页Fudan University Journal of Medical Sciences

基　　金：国家自然科学基金资助项目 ( 3 95 70 698)

摘　　要：目的　用90 钇 (90 Y)标记抗肺癌单克隆抗体LC1IgM ,研究放射免疫导向药物局部用药对荷肺癌裸鼠的治疗作用 ,判断其疗效。方法　将抗肺癌单克隆抗体LC1IgM与90 Y耦合 ,制成放射免疫导向药物。将皮下已接种肺癌组织的裸鼠按药物不同放射剂量随机分成对照、3.7、7.4、11.1、14 .8和 2 2 .2MBq 6个组。对荷肺癌裸鼠进行肿瘤局部注射90 Y LC1。给药当日及给药后 4周内每周测定肿瘤大小 ,计算肿瘤抑制率 ,与对照组进行比较。结果　 7.4MBq组与对照组之间存在显著统计学意义 (P <0 .0 1)。 11.1、14 .8MBq组与对照组之间的差异有统计学意义 (P <0 .0 5 ) ,而 3.7MBq组与对照组及各治疗组之间的治疗效果无统计学意义 (P >0 .0 5 )。当剂量大于 11.1MBq时 ,局部给药可引起裸鼠死亡。结论　放射性核素90 Y标记抗肺癌单克隆抗体LC1局部治疗可抑制荷肺癌裸鼠的肿瘤生长。放射剂量过大 (>11.1MBq)可产生毒性。90 YPurpose: To label monoclonal antibody LC1 with 90Y. To investigate and evaluate the topical curative effect of radioimmunological targeting drug on nude mice with lung cancer. Methods: Combine anti-lung cancer monoclonal antibody LC1 IgM with 90Y to produce radioimmunological targeting drug. The nude mice which had been inoculated subcutaneously with lung cancer tissue were randomized into 6 groups according to different dosage of the durg: the control group, 3. 7,7. 4,11. 1,14. 8 and 22. 2 MBq group. The size of the tumor was measured just before treatment and every week after treatment in four weeks. The inhibition rate (IR) was calculated and compared with the control group. Results: There was highly statistical significance between 7.4 MBq group and control group (P 0.0 5). A dosage greater than 11.1 MBq would cause nude mice die. Conclusions: Anti-lung cancer monoclonal antibody LC1 labeled with radionuclide 90Y could inhibit the growth of the tumor when given topically. It would result in toxicity if the dosage was too great (greater than 11.1 MBq). 90Y-LC1 has a potential value of clinical application.

关 键 词：肺癌 单克隆抗体 ^90钇 放射免疫治疗 

分 类 号：R734.2[医药卫生—肿瘤]