Increase of β -1, 3-Glucanase and Chitinase Activities in Cotton Callus Cells Treated by Salicylic Acid and Toxin of Verticillium dahliae  

水杨酸和黄萎病菌毒素处理棉花愈伤组织使β-1,3-葡聚糖酶和几丁质酶的活性增加(英文)

作　　者：李颖章[1] 郑晓华[1] 唐海林[1] 朱建伟[1] 杨晶明[1] 

机构地区：[1]中国农业大学生物学院

出　　处：《Acta Botanica Sinica》2003年第7期802-808,共7页Acta Botanica Sinica（植物学报：英文版）

基　　金：国家自然科学基金(30170555);教育部高校骨干教师资助项目~~

摘　　要：The different resistance of cotton (Gossypium hirsutum L.) cultivars to crude toxin of Verticillium dah/iae(VD) was correlated with the activities of chitinase and β-1, 3-glucanase in callus cells. The activities of chitinase and β-1, 3-glucanase in the callus cells treated with the VD-toxin were increased to the higher level at earlier time point in resistant cultivars than these in the susceptible cultivars. Exogenous salicylic acid (SA) induced the accumulation of chitinase and β -1,3-glucanase, which resulted in the resistance of callus cells to the VD. toxin. Western blot using a polyclonal antibody against β -1,3-glucanase identified 28 kD protein that was induced by VD-toxin, SA, or VD-toxin plus SA.不同品种棉花(Gossypium hirsutum L.)愈伤组织对黄萎病菌毒素粗提物的抗性与体内β-1,3-葡聚糖酶和几丁质酶活性水平有关。在毒素处理下,抗性品种比感性品种酶活性增加的幅度大、时间早、外源水杨酸(SA)处理后,棉花愈伤组织中的β-1,3-葡聚糖酶和几丁质酶活性增加。抗β-1,3-葡聚糖酶多克隆抗体与28kD的蛋白条带有免疫交叉反应,毒素、SA、毒素+SA均能诱导该蛋白条带出现。

关 键 词：Gossypium hirsutum toxin of Verticillium dahliae salicylic acid CHITINASE Β-1 3-GLUCANASE 

分 类 号：S435.62[农业科学—农业昆虫与害虫防治]