荧光原位杂交检测人与棕色田鼠Y染色体  

Detection of Y Chromosome in Human and Microtus mandarinus with FISH

作　　者：朱必才[1] 高建国[1] 张子峰[1] 张永[1] 高俊芳[1] 侯进慧[1] 

机构地区：[1]徐州师范大学生物系,江苏徐州221116

出　　处：《遗传》2003年第5期517-520,共4页Hereditas（Beijing)

基　　金：国家自然科学基金资助项目(39970405;30270738);徐州师范大学学工处基金资助

摘　　要：应用荧光原位杂交技术,以人Y染色体长臂上q12区的高度重复序列DNA探针pY3.4,分别与人和棕色田鼠的中期染色体标本及间期细胞核进行杂交,检测人和棕色田鼠的Y染色体.结果显示,人的中期染色体上和间期细胞核中发现了杂交信号,棕色田鼠的未发现杂交信号,由此讨论了棕色田鼠DNA与人Y染色体pY3.4的同源性和棕色田鼠的FISH结果,以便进一步研究棕色田鼠Y染色体。The purpose of the investigation was to detect the Y chromosome of human and Microtus mandarinus with the satellite DNA probe in human′s Yq by fluorescence in situ hybridization(FISH). We hybridizied the probe with the metaphase chromosome and interphase nucleus specimens of humanbeing and Microtus mandarinus respectively.The results showed that there was hybridization signal on metaphase chromosome and interphase nucleus of human,but no signal on metaphase chromosome and interphase nucleus of Microtus mandarinus.According to the results,we discussed the homologue between pY3.4 in Y chromosome of humanbeing and DNA of Microtus mandarinus and FISH result of Microtus mandarinus.

关 键 词：荧光原位杂交 棕色田鼠 人 Y性染色体 

分 类 号：Q953[生物学—动物学] Q959.837