RASSF1 A转录本在肺癌组织中表达及其临床意义  

Transcriptional inactivation of RASSF1A in lung cancer and its clinical significance

作　　者：邵康[1] 肖智雄[2] 唐槐静[3] 程邦昌[1] 张德超[3] 周芳[3] 熊美华[3] 姆巴东[1] 黄微[3] 张翠艳[3] 赫捷[3] 

机构地区：[1]武汉大学人民医院胸心外科,430060 [2]美国波士顿大学医学院生化系与医学系 [3]中国医学科学院中国协和医科大学肿瘤研究所肿瘤医院胸外科

出　　处：《中华医学杂志》2003年第7期569-571,共3页National Medical Journal of China

基　　金：国家科技攻关计划项目基金资助 ( 2 0 0 2BA711A0 6)

摘　　要：目的　探讨RASSF1A(RAS相关区域家族 1A)基因转录表达与肺癌发生、发展的关系。方法　采用逆转录 聚合酶链反应 (RT PCR)的方法检测 4 7例肺癌患者癌组织及相应癌周正常组织中RASSF1AmRNA的表达水平。结果　 (1)RASSF1AmRNA在所有正常组织中表达 ,而在肺癌癌组织中存在较高的表达缺失率 (5 3 2 % )。 (2 )淋巴结转移阳性者癌组织中RASSF1AmRNA表达缺失率 (70 4 % )明显高于淋巴结转移阴性者 (30 0 % ) (P <0 0 1) ;(3)RASSF1AmRNA表达缺失率与肺癌分期有关 ,越晚病例RASSF1A表达缺失率越高 (P <0 0 5 ) ;(4 )RASSF1AmRNA表达缺失与肿瘤组织学类型、肿瘤分化程度及吸烟指数未见显著相关 (P >0 0 5 )。结论　RASSF1A转录表达缺失 ,它可能参与调控肺癌的发生、发展过程 ,并进而影响其生物学特点及预后。Objective To explore the relationship between the transcriptional expression of RASSF1A (Ras association domain family 1A gene) and oncogenesis and development of lung cancer. Method RT-PCR was used to detect the expression of RASSF1A mRNA in 47 human lung cancer tissues and matched 47 non-cancer tissues. Results (1). The RASSF1A mRNA was identified in all non-cancer tissues but not found in 53.2% carcinoma tissues. (2). The rate of loss of RASSF1A mRNA was significantly higher in the patients with positive lymph node metastasis (70.4%) than in those without lymph node metastasis (30.0%) ( P <0.01). (3). Much more frequent in advanced tumor tissues, loss of RASSF1A mRNA was correlated with TNM stage ( P <0.05). (4). No significant association of abnormal RASSF1A expression was identified with histological type, differentiation grade of tumors or age, sex, and smoking index of the patients (all P >0.05). Conclusion RASSF1A is one of the new candidates of tumor suppressor genes. Loss or abnormal down-regulation of RASSF1A mRNA is a frequent event in lung carcinogenesis, which may play an important role in the malignant progression and prognosis of lung cancer.

关 键 词：RASSF1A 转录本 肺癌 表达 癌组织 

分 类 号：R734.2[医药卫生—肿瘤]