微粒体环氧化物水解酶基因多态性与慢性阻塞性肺疾病的关系  

Association between polymorphisms in the microsomal epoxide hydrolase (mEH) gene and chronic obstructive pulmonary disease

作　　者：肖丹[1] 王辰[1] 杜敏捷[1] 庞宝森[1] 张洪玉[1] 肖白[2] 刘敬忠[2] 翁心植[1] 苏丽[3] David C.Christiani 

机构地区：[1]首都医科大学附属北京朝阳医院-北京呼吸疾病研究所 [2]首都医科大学基础医学研究中心,北京100020 [3]哈佛大学公共卫生学院环境保护系职业病研究科

出　　处：《中华医学杂志》2003年第20期1782-1786,共5页National Medical Journal of China

基　　金：国家"十五"科技攻关课题资助项目(2 0 0 1BA70 3B0 3 ) ;北京市自然科学基金资助项目 (70 2 2 0 14 )

摘　　要：目的　明确微粒体环氧化物水解酶 (mEH)基因多态性与中国人慢性阻塞性肺疾病(COPD)易感性的关系。方法　用聚合酶链反应 限制性片段长度多态性 (PCR RFLP)分析的方法检测 10 0对年龄、性别匹配的COPD患者和健康对照者mEH外显子 3(Tyr113→His)、外显子 4 (His139→Arg)基因多态性。结果　 (1)COPD组有 4 2例 (42 % ) ,对照组有 32人 (32 % )为mEH外显子 3的His139突变杂合子 (P =0 .0 15 ) ,经年龄、性别、体块指数和吸烟年支数调整后的OR值为 2 .96 (95 %CI为 1.2 4～ 7.0 9) ;(2 )极慢代谢活性的mEH基因型对应其他代谢活性基因型在病例组和对照组间未发现明显差别 ;(3)将病例组分为不吸烟者和吸烟者 (包括目前仍吸烟者和戒烟者 )两组 ,极慢代谢活性的mEH基因型对应其他代谢活性基因型在不吸烟者OR值均大于 1.0 0 ,而在吸烟者中均小于1.0 0。结论　 (1)mEH外显子 3的His113突变杂合子与中国COPD易感性有关 ;(2 )COPD患者mEH基因型与吸烟之间可能存在交互作用。Objective To investigate the association between polymorphisms in the microsomal epoxide hydrolase (mEH) gene and susceptibility to chronic obstructive pulmonary disease (COPD) in a Chinese population. Methods Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) were performed to genotype mEH polymorphisms in exon3 (Tyr113→His) and exon4 (His139 →Arg) in 100 COPD patients and 100 age and sex matched healthy controls. Results (1) The proportion of mEH heterozygotes in exon3 was significantly higher in the patients with COPD than that in the control subjects (42% vs 32%). The odds ratio ( OR ) adjusted by age, sex, body mass index (BMI) and cigarettes years was 2.96(95% CI 1.24～7.09). (2) There was no marked difference in very slow activity genotype versus other genotypes between COPD patients and controls. (3) When COPD patients were nonsmokers, the OR of very slow activity genotype versus other genotypes was more than 1.00, and when COPD patients were smokers (Current smokers and ex-smokers), the OR was less than 1.00. Conclusions (1) mEH heterozygotes in exon3 might be associated with the susceptibility to COPD in China. (2) The interaction might be existed between mEH genotype and smoke.

关 键 词：微粒体 环氧化物水解酶 基因多态性 慢性阻塞性肺疾病 吸烟 基因 

分 类 号：R563.9[医药卫生—呼吸系统]