树突状细胞负载肝癌抗原肽疫苗体外诱导特异性免疫学反应的研究  

Study on immunoreaction induced by DCs loaded with hepatocarcinoma antigen peptide in vitro

作　　者：曲萍[1] 隋延仿[1] 郭爱林[1] 曹云新[2] 董海龙[1] 禄韶英[3] 叶菁[1] 张秀敏[1] 

机构地区：[1]第四军医大学病理学教研室,陕西西安710032 [2]第四军医大学免疫学教研室,陕西西安710032 [3]西安交通大学第一医院普外科,陕西西安710061

出　　处：《细胞与分子免疫学杂志》2003年第4期406-408,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金重点项目资助 (No.39830 4 2 0 )

摘　　要：目的 :研究树突状细胞 (dendriticcell,DC)负载肝癌抗原肽EPVTKAEML体外诱导特异性CTL的能力及其抑癌效果。方法 :用顺序特异引物聚合酶链反应技术 (PCR SSP)选择HLA B7表型供者 ,从脾组织中分离、培养DC EPVTKAEML特异性CTL。用51Cr释放法检测CTL的杀伤活性 ,并用抗HLA I分子单抗 (mAb)进行杀伤抑制实验。结果 :找到 4例HLA B7杂合子供者 ,用DC负载HLA B7限制的抗原肽EPVTKAEML可诱导特异性CTL反应 ,对肝癌细胞HHCC有较强的杀伤作用。结论AIM: To investigate the potency and antitumor effect of specific cytotoxic T lymphocytes induced by hepatocarcinoma antigen peptide EPVTKAEML loaded dendritic cells (DCs). METHODS: The healthy donors of HLA B7 genotype were selected by PCR SSP. Isolated and cultured DCs from HLA B7 donors’ spleen tissues were loaded with the peptide EPVTKAEML from hepatocarcinoma cell line HHCC. The specific CTL reaction and inhibitory effect of anti HLA I molecule mAb on CTL cytotoxicity were detected in vitro by 51 Cr release assay. RESULTS: 4 donors with HLA B7 genotype were found. Their spleen T lymphocytes could be induced to proliferate and differentiate into specific CTLs by DCs loaded with the peptide. Antibody blocking test demonstrated that the cytotoxity of CTLs was strictly limited by MHC I molecule. CONCLUSION: EPVTKAEML loaded DCs can induce efficient and specific antitumor immune reaction in vitro .

关 键 词：树突状细胞疫苗 抗原肽 肝癌 HLA-B7 

分 类 号：R735.7[医药卫生—肿瘤] R392.12[医药卫生—临床医学]