维司力农对缺血再灌注大鼠心肌NO含量及PKC活性的影响  

Effect of vesnarinone on PKC activity and NO content on myocardial injury induced by ischemia and reperfusion in rats

作　　者：谢文利[1] 朱江[1] 晋玉章[1] 

机构地区：[1]武警医学院药理学教研室,天津300162

出　　处：《中国药理学通报》2003年第5期563-564,共2页Chinese Pharmacological Bulletin

基　　金：武警总部科学研究项目资助课题;NoWY2 0 0 0 5 3

摘　　要：目的　探讨维司力农对缺血再灌注心肌的保护作用机制。方法　采用NO和PKC测定试剂盒分别测定了两个对照组、心肌缺血预适应组、不同剂量维司力农组的大鼠心肌细胞内一氧化氮 (NO)含量和蛋白激酶C(PKC)活性。结果　高、低剂量维司力农组PKC活性高于两个对照组 ;低剂量组PKC活性与缺血预适应组相当 ,高剂量组PKC活性明显高于缺血预适应组。同时 ,NO含量也高于两个对照组和缺血预适应组。结论　维司力农可使大鼠心肌细胞内NO含量和PKC活性提高 ,由此推断 :维司力农对缺血再灌注心肌的保护作用很可能是通过NO激活PKC而实现的。AIM To study the protective effects of vesnarinone on myocardial ischemia. METHODS Rat myocardial oxide nitric (NO) content, protein kinase C (PKC) activities in different groups were determined by kits. RESULTS Vesnarinone 1 mg·kg -1 or 2 mg·kg -1 iv markedly improved the NO content and PKC activity before ischemia for 30 min and reperfusion for 2 h in rats. CONCLUSION Vesnarinone possesses a protective effect against myocardial ischemia via NO induce PKC.

关 键 词：维司力农 心肌缺血预适应 一氧化氮 蛋白激酶C 

分 类 号：R-332[医药卫生] R284