甲壳胺体外对小鼠巨噬细胞PKC和DAG的作用研究  

Effect of chitosan on PKC activity and DAG concentration in murine peritoneal macrophages

作　　者：梁东升[1] 李明春[1] 安丰妹[2] 王月军[1] 袁锦华[1] 

机构地区：[1]解放军第401医院药剂科,青岛266071 [2]解放军第401医院全军手外科中心,青岛266071

出　　处：《中国药理学通报》2003年第6期643-645,共3页Chinese Pharmacological Bulletin

基　　金：军队"十五"医药卫生科研基金;No 01MB047

摘　　要：目的 观察甲壳胺体外对小鼠腹腔巨噬细胞蛋白激酶C(PKC)活性和二酰基甘油(DAG)合成的影响。方法分别采用反相离子对高效液相色谱法与放射免疫测定技术测定PKC活力和DAG含量。结果 ①甲壳胺剂量依赖性引起巨噬细胞中PKC活性明显增强并使之发生质膜转位,达峰时间为25min,1.5 h恢复到基础水平;②甲壳胺促进巨噬细胞中DAG的合成,达峰时间为30 s,3 min恢复到基础水平。结论 甲壳胺的免疫增强作用与其增强巨噬细胞中PKC活性和DAG合成有关,DAG/PKC信号途径参与了其对巨噬细胞的活化过程。ABSTRACT AIM To investigate the effect of ch-itosan on protein kinase C (PKC) activitives and diacyl glycerol(DAG)concentration in murine peritoneal macrophages. METHODS A new ion-pair reversed-phase high liquid chromatography and ra-dioimmunoassay were used to determinate the activity of PKC and the concentration of DAG respectively. RESULTS ( 1) Chitosan induced activation and translocation of PKC in murine peritoneal macrophages. The peak time was 25 min and the activity of PKC came back the basic level at 1. 5 h. (2)Chitosan increased the production of DAG in murine peritoneal macrophages. The peak time was 30 s and the concentration of DAG came back the basic level at 3 min. CONCLUSION The im-munopoteniating effect of chitosan may be associated with the channel of DAG /PKC.

关 键 词：甲壳胺 巨噬细胞 蛋白激酶C 二酰基甘油 信号转导 

分 类 号：R-332[医药卫生] R284.1