共生小球藻对宿主绿草履虫形态、生长、抗氧化能力及细胞质糖含量的影响  

Effects of Symbiont on the Morphology,Growth,Resistance to Oxidation,and Saccharide Content of Hosts in the Paramecium bursaria-Chlorella System

作　　者：孙军[1] 翟万银[1] 丁祥欢[2] 顾福康[1] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]华东师范大学化学系,上海200062

出　　处：《Zoological Research》2003年第3期191-195,共5页动物学研究（英文）

基　　金：国家自然科学基金资助项目 ( 3 9970 0 88);高等学校博士学科点专项基金资助项目

摘　　要：以含共生藻绿草履虫 (CCP)、恢复型绿草履虫 (RCCP)和无共生藻绿草履虫 (CFP)为材料 ,探讨共生体对宿主生长、细胞大小、抗氧化能力和细胞质糖含量的影响。 3种细胞培养液中 ,细胞培养密度峰值分别为 15 0 0、 80 0和 5 0 0个 /mL ,对应的生长峰值期分别在第 14天、第 14天和第 8天。CCP细胞比CFP约长 2 2μm ,约宽 16 μm ;RCCP在CCP和CFP之间。对邻苯三酚自氧化产生O2 化学发光的抑制效果不同 :随匀浆液浓度 (μL/mL)从 2 0增加到 10 0时 ,CCP抑制率提高了 6 7% ,RCCP提高了 4 2 % ,而CFP和小球藻仅分别为 2 7%和<10 %。细胞质葡萄糖和麦芽糖含量差异巨大 :CCP分别为RCCP的 4和 2倍 ,而CFP是RCCP的 1/ 4和 1/ 2。看来 ,绿草履虫在这个共生系统中受益。Using Chlorella-containing Paramecium bursaria (CCP),recovered Chlorella-containing P.bursaria (RCCP) and Chlorella-free P.bursaria (CFP) as materials,the effect of symbiotic Chlorella on the cell size,growth,resistance to oxidation,and saccharide content of the host cells were measured.The highest density of cells in the three cultures was 1?500,800,500 cells/mL,respectively,corresponding to the peak period of growth on day 14,14 and 8.CCP cells averaged 22?μm longer and 16?μm wider than CFP ones,and those of RCCP cells fell between CCP and CFP.The resistance to O 2 - chemiluminescence produced by self-oxidation of pyrogallol was different:when concentrations of the cytoplasm homogenate increased from 20 to 100?μL/mL,CCP increased for 67%,RCCP for 45%,CFP for 27%,and the symbiotic Chlorella for <10%.Of glucose and maltose in cytoplasm,CCP contents were 4 and 2 times higher than those in RCCP,respectively,and CFP were only 1/4 and 1/2 of RCCP.It is very likely that paramecium-hosts benefit from the symbiotic system.

关 键 词：绿草履虫 小球藻 共生系统 形态 生长 糖 抗氧化能力 

分 类 号：Q958[生物学—动物学]