肿瘤成纤维细胞促进血管形成能力的研究  

Study on the angiogenic ability of tumor fibroblasts

作　　者：朱红梅[1] 汤为学[2] 周卫平[1] 

机构地区：[1]重庆医科大学病毒性肝炎研究所,400010 [2]重庆医科大学病理生理学教研室

出　　处：《中华肝脏病杂志》2003年第12期735-738,共4页Chinese Journal of Hepatology

摘　　要：目的 探讨成纤维细胞在肿瘤血管形成中的作用。方法 对肿瘤成纤维细胞模型L_(929)—H_(22)细胞,用免疫细胞化学测定其促进血管形成因子的表达、双室联合培养测定其促进内皮细胞形成管型的能力与促进肿瘤细胞侵袭的能力,分析其粘贴能力、条件培养基(CM)对H_(22)细胞生长的影响,并与其亲代细胞L_(929)比较。结果 与L_(929)细胞相比,L_(929)—H_(22)细胞粘贴能力增强(F≥104.32,P<0.001);表达血管形成因子基质金属蛋白酶9(MMP—9)、转化生长因子β_1(TGF-β_1)、细胞粘合素(TN)、bcl—2增强(t≥3.305 5,P<0.01);更能促进肿瘤细胞侵袭(F=266.30,P<0.001)与ECV304细胞形成管型(q≥3.2289,P<0.01)。其CM对H22细胞生长有一定的促进作用(F=19.41,P<0.05)。结论 肿瘤成纤维细胞表达血管形成因子增强,在肿瘤侵袭与血管形成中有一定作用。Objective To explore the role of fibroblasts derived from tumor in the tumor angiogenesis. Methods Two-well co-culture system were used to detect the expression of MMP-9, TGF- ft, TN and bcl-2 in L929-H22 cells, and their ability of promoting angiogenesis of ECV304 cells and invasion of MDA-MB-231 cells respectively, which were established in our laboratory before. Then their adhesion and the effect of their supernatant on H22 cells proliferation were analysed. Results Compared with L929 cells, the adhesion potential of L929-H22 cells increased (F≥104.32, P < 0.001), with the higher level of expression of MMP-9, bcl-2, TN, and TGF- ft in L929-H22 cells in creased (t ≥3.305 5, P < 0.01). L929-H22 and L929 cells enhanced the invasiveness of human mammary cancer MDA-MB-231 cells through artificial basement membrane (Matrigel) 1.21 and 0.48 times respectively (F =266.3, P < 0.001). L929-H22 cells induced morphogenesis of ECV304 cells. L929-H22 stimulated endothelial cells to form more and longer tubes than L929 did (F≥ 23.75, P < 0.01). 25% CM of L929-H22 cells stimulated the growth of H22 cells (F = 266.30, P < 0.05). Conclusion The results suggested that fibroblasts in tumors secrete more growth factors and angiogenic factors to promote the angiogenesis and invasion of solid tumors.

关 键 词：肿瘤 成纤维细胞 血管形成 免疫细胞化学 肿瘤侵袭 

分 类 号：R730.2[医药卫生—肿瘤]