肝癌组织碱性磷酸酶糖链结构的研究  

Changes of alkaline phosphatase sugar chains in hepatocellular carcinoma tissue

作　　者：陈国千[1] 章清 许艳芳[3] 张万忠[1] 关明[1] 苏兵[1] 梁惠琪[1] 吕元[1] 

机构地区：[1]复旦大学附属华山医院检验科,上海200040 [2]上海市第五医院 [3]温州医学院检验系

出　　处：《中华肝脏病杂志》2003年第12期739-741,共3页Chinese Journal of Hepatology

摘　　要：目的 探讨肝癌组织碱性磷酸酶(ALP)N-糖链结构的改变及与肝癌侵袭性的关系。方法 用凝集素亲和层析法检测9例正常肝组织、16例肝癌组织和16例肝癌癌旁组织中ALP的多种凝集素结合率。凝集素包括L型红腰豆凝集素(L-PHA)、小扁豆凝集素(LCA)、蔓陀萝凝集素(DSA)、E型红腰豆凝集素(E-PHA)和黑接榾凝集素(SNA)等。结果 肝癌组织ALP的L-PHA、LCA、DSA、E-PHA和SNA结合率分别为22.94％±5.30%、55.97％±13.72％、38.16％±8.87％、11.56％±4.81％和69.80％±13.71％,均明显高于正常肝组织(5.89％±2.75％、36.20％±11.58％、17.90％±6.71％、5.38％±2.20％和57.32％±11.27%),t值分别为8.94、3.64、5.94、3.62和2.32,P值均<0.01;侵袭性肝癌组织ALP的L-PHA结合率(25.84％±4.67％)明显高于非侵袭性肝癌组织(18.10％±3.64％),t 3.71,P<0.01。结论 肝癌组织ALP的糖链结构发生改变,且ALP的L-PHA结合率与肝癌侵袭性有关。Objective To investigate the changes of sugar chain structures of alkaline phosphatase (ALP) in hepatoma tissue and its relation to the invasiveness of hepatocellular carcinoma (HCC). Methods The binding ratios of ALP from 9 normal liver tissues,16 hepatoma tissues and 16 noncancerous tissues surrounding hepatoma were analysed by affinity chromatography on various lectin columns including leukoagglutinating phytohemagglutinin (L-PHA),lentil lectin (LCA),Datura stramonium agglutinin (DSA),erythroagglutinating phytohemagglutinin (E-PHA) and Sambucus nigra bark agglutinin (SNA). Results The binding ratios of ALP on L-PHA (22.94% ± 5.30%),DSA (55.97% ± 13.72%), LCA (38.16% ± 8.87%),E-PHA (11.56% ± 4.81%) and SNA (69.80%± 13.71%) in HCC tissues were significantly increased (P<0.01) compared with that in normal liver tissues (L-PHA 5.89%± 2.75%,DSA 36.20%± 11.58%,LCA 17.90% ± 6.71%,E-PHA 5.38% ± 2.20%,SNA 57.32% ± 11.27%),respectively,t values between the two groups were 8.94,3.64,5.94,3.62 and 2.32,respectively. L-PHA-binding ratio (25.84% ± 4.67%) of ALP in HCC with invasiveness was significantly higher than that (18.10% ±. 3.64%) without invasiveness (t= 3.71,P<0.01). Conclusions The changes of ALP sugar chain structures occur in HCC tissue,b1-6 branching sugar chain structure of ALP is related to the invasiveness of HCC.

关 键 词：肝癌 碱性磷酸酶 肿瘤侵袭 糖链结构 肿瘤标志物 

分 类 号：R735.7[医药卫生—肿瘤]