低氧对大鼠肝肾组织内红细胞生成素和低氧诱导因子-1α基因表达的影响  

Effects of acute hypoxia and intermittent hypoxic acclimatization on erythropoietin and hypoxia-inducible factor-1α gene expression in rat hepatic and renal tissues

作　　者：陈伟[1] 陈家佩[2] 葛世丽[2] 从玉文[2] 付小兵[1] 

机构地区：[1]解放军第三○四医院全军创伤修复重点实验室,北京100037 [2]军事医学科学院放射医学研究室

出　　处：《中国危重病急救医学》2004年第1期9-12,共4页Chinese Critical Care Medicine

基　　金：国家自然科学基金重点课题资助项目 ( 3 973 0 190 );国家重点基础研究规划项目 ( G19990 5 42 0 4)

摘　　要：目的　观察急性低氧和间断低氧习服对大鼠肝、肾组织内红细胞生成素 (EPO)及其调控蛋白低氧诱导因子 1α(HIF 1α)基因表达的影响。方法　用低压舱模拟高原低氧环境 ,进行 30 0 0 m 2周、5 0 0 0 m2周低氧训练 ,使大鼠达到低氧习服状态后 ,采用 Northern斑点杂交研究间断低氧习服前后 ,80 0 0 m4 h低氧处理的大鼠肝、肾组织内 EPO和 HIF 1α基因表达的变化。结果　与常氧对照组相比 ,急性低氧处理的大鼠肝、肾组织内 EPO基因表达都明显增强 (P<0 .0 5或 P<0 .0 1) ;而间断低氧习服后 ,同样的低氧处理的大鼠肝、肾组织中 EPO基因表达都明显低于急性低氧组 (P<0 .0 1) ,EPO m RNA含量与常氧对照组水平差异不显著 (P>0 .0 5 )。急性低氧处理的大鼠肝组织中 HIF 1α基因表达水平明显高于常氧对照组和间断低氧习服组 (P均 <0 .0 1) ,而在不同处理的肾组织中 ,该基因表达水平差异不明显 (P均 >0 .0 5 )。结论　急性低氧能够刺激肝、肾组织内 EPO基因表达 ;而间断低氧习服的大鼠耐低氧能力明显增强 ,肝、肾组织内 EPO基因表达水平可恢复到常氧对照组水平。 HIF 1α在低氧影响 EPO基因表达的过程中可能起着重要的调节作用。Objective To explore the effects of acute hypoxia and intermittent hypoxic acclimatization on gene expression of erythropoietin (EPO) and hypoxia-inducible factor-1α (HIF-1α) in rat hepatic and renal tissues. Methods Twenty-four rats (weight was 180 to 220 g) were divided into three groups: normal control group (NC), intermittent hypoxic acclimatization (IH) and acute hypoxia groups (AH). Gene expression of EPO and HIF-1α were examined with Northern dot blot method. Results As compared with NC group, the levels of EPO gene expression in rat hepatic and renal tissues in AH group were both significantly elevated (P<0.05 or P<0.01). In IH group, the contents of EPO mRNA in hepatic and renal tissues were apparently decreased versus AH group (P<0.01), whilst were not differently elevated in comparison with group NC (P>0.05). In hepatic tissue from AH group, the content of HIF-1α mRNA were more than those in NC group and IH group, while the levels of gene expression of HIF-1α were no substantial change in renal tissues from different groups (all P>0.05). Conclusion Hypoxic acclimatization can inhibit the increment of EPO gene expression induced by acute hypoxia in rat hepatic and renal tissues, in which HIF-21α may play important roles.

关 键 词：低氧 急性 间断低氧习服 红细胞生成素 低氧诱导因子-1Α 

分 类 号：R363[医药卫生—病理学]