南美白对虾(Penaeus vannamei)视神经节和脑免疫细胞化学研究  

IMMUNOCYTOCHEMICAL STUDIES ON THE OPTIC GANGLIA AND THE BRAIN OF PENAEUS VANNAMEI

作　　者：叶海辉[1] 王桂忠[1] 金朱兴[1] 李少菁[1] 黄辉洋[1] 陈锦民[1] 

机构地区：[1]厦门大学海洋系亚热带海洋研究所

出　　处：《海洋与湖沼》2004年第1期78-83,共6页Oceanologia Et Limnologia Sinica

基　　金：福建省重中之重"福建省海洋生物优良种质和生物活性物质的应用基础研究"项目 (1 998- 2 0 0 2 )资助

摘　　要：应用抗脊椎动物 5 羟色胺、神经肽Y、β 内啡肽、P物质、生长抑素、胰高血糖素、催乳素抗体和SABC免疫细胞化学方法 ,对南美白对虾视神经节和脑进行了免疫细胞化学研究。结果表明 ,前 4种抗体呈现免疫阳性反应 :5 羟色胺免疫阳性细胞分布于视神经节的视神经层、视外髓、视内髓和视端髓 ;前脑前中细胞群 ,中脑嗅叶内侧细胞群、嗅叶外侧细胞群以及前脑中部的前端神经髓质呈免疫阳性反应。神经肽Y免疫阳性细胞分布于视神经节的 4个神经髓 ;前脑前中群细胞和前脑中部的前端神经髓质也呈阳性反应。P物质免疫阳性细胞位于前脑前中群。β 内啡肽免疫阳性细胞仅分布于后脑后侧群。 4种免疫阳性物质特异性的分布模式 。Amines and peptides are important substances of biological activity in crustaceans. In the life process they play the role of hormone, neurotransmitter and/or neuromodulator, the optical ganglia and the brain are the center of neuroendocrinology in crustaceans, exerting significant modulation in molting, reproduction and other physical activities. Penaeus vannamei, despite being of global commercial importance, has never been subject to neuroendocrinological research. In this paper, immunocytochemistry techniques were used to observe the immunoreactive neurons and neuropils in the optic ganglia and brain of P . vannamei. The sections were stained with antibodies of serotonin (5 HT), neuropeptide Y (NPY), β Endorphin(β Ep), substance P (SP), somatostatin (SS), glucagon (Glu) and proctolin (PRL). The immunostained sections were observed and the positive neurons and neuropils were examined under a light microscope. Immunoreativities are found against 5 HT, NPY, β Ep and SP. Their distributions are as following: 5 HT immunoreactive cells are located in lamina ganglionaris (LG), medulla externa (ME), medulla interna (MI) and medulla terminalis (MT). The immunoreactivity of 5 HT was detected in the anterior median cluster, the anterior medial protocerebrum neuropiles, the inner and outer lateral cluster to olfactory lobe of the deutocerebrum. The presence of NPY immunoreactive cells was found in the four neuropils of the optic ganglia. The immunoreactivity of NPY was located in the anterior median cluster and the anterior medial protocerebrum neuropiles. SP immunoreactive cells and β Ep immunoreactive cells existed in the anterior median cluster of protocerebrum and the posterior lateral cluster of tritocerebrum respectively. The wide distribution of 5 HT immunoreactivity in the optical ganglion of P. vannamei suggests that 5 HT probably plays the role of neuromodulator in the nerve cells. The 5 HT immunoreactivity in the cerebrum of P. vannamei may supply morphological proofs for

关 键 词：南美白对虾 视神经节 脑 免疫细胞化学 

分 类 号：Q952[生物学—动物学]