选育蒽降解微生物耦合系统菌株的电融合及融合子的筛选  

Elertrofusion of Building Coupling System for Degrading Anthracene and Fusants' Choices

作　　者：张甲耀[1] 季淼[2] 方呈祥[1] 陈菲莉[1] 陈玲[1] 邓欢欢[1] 

机构地区：[1]武汉大学资源环境学院环境科学系,武汉430072 [2]合肥工业大学生物与食品工程学院,合肥230009

出　　处：《环境科学》2004年第1期49-52,共4页Environmental Science

基　　金：国家自然科学基金项目 (编号 :2 0 0 770 2 1)

摘　　要：对选育蒽降解微生物耦合降解系统菌株的电融合过程及融合子的筛选进行研究 .结果发现在 5 0 0kHz的交流频率 ,脉冲强度 30 0V/cm ,脉冲幅宽为 5 μs ,脉冲个数为 2 ,交流电场强度在 2 0～ 4 0V/cm范围内 ,总融合率较稳定 .可以用红霉素和氨苄青霉素的双抗平板来对表面活性剂产生菌与土生优势菌的融合子进行筛选 ,用含卡那霉素的蒽的高渗平板对蒽的高效降解菌和土生优势菌的融合子进行筛选 .2种融合子均可以用绿色荧光来确证 .Some researches have been carried out for the electro fusion of building coupling system for degrading anthracene and the choices of fusants. During the experiments, total rate of fusion stable in alternation current frequency 500kHz,impulse intensity 300V/cm, impulse width 5μs,amount of impulse 2 and alternation current intensity 20～40 V/cm were found. The resistant plate with erythromycin and ampicillin can screen the fusant of autochthonous bacterium and surfactant-producing bacterium. The resistant plate with anthracene and karnamicin can screen the fusant of autochthonous bacterium and efficient anthracene-degradation bacterium. The new fusants were found to have stable fluorescent characteristics .

关 键 词：融合子 耦合系统 电融合技术 抗性 高效降解菌 微生物 

分 类 号：X172[环境科学与工程—环境科学]