北京地区人乳头瘤病毒16 E6E7基因变异和序列分析  

The Mutations and Sequencing of HPV16E6E7Gene of Human Papillomavirus from Patients with HPV Infection in Beijing

作　　者：左亚刚[1] 王家璧[1] 许雪梅[2] 朱明昭[2] 刘方[1] 司静懿[2] 宋国兴[2] 

机构地区：[1]中国医学科学院,中国协和医科大学北京协和医院皮肤科,北京100730 [2]中国协和医科大学基础医学研究所生物物理室

出　　处：《中华皮肤科杂志》2003年第11期650-652,共3页Chinese Journal of Dermatology

摘　　要：目的了解北京地区人乳头瘤病毒(HPV)16型E6E7结构特点,为研制HPV16疫苗奠定基础。方法从HPV感染组织中提取DNA,PCR鉴别其型别,从单独HPV16感染的标本中分离HPV16E6E7基因,克隆入载体pGEM-3ZF,进行双向测序,与德国标准株进行比较。结果构建了北京地区HPV16E6E7的重组质粒。测序结果表明该基因全长为776bp,与已发表的德国标准株长度相等,3处核苷酸发生变异,同源性99.7%。分别位于第60、96和565nt(相当于HPV16全长序列中第142、178和647nt),2处位于E6区,1处位于E7,其对应氨基酸分别为第20、32和188个氨基酸,分别由Pro(CCA)、Asp(GAT)和Asn(AAT)变异为Pro(CCG)、Glu(GAG)和Ser(AGT)。结论北京地区HPV16E6E7基因结构与德国标准株存在差异。Objective To investigate the gene sequence and mutations of human papillomavirus(HPV)type16E6E7in patients with HPV infection in Beijing.Methods Sample DNA was extracted from lesions in patients with HPV infection.HPV types were identified by polymerase chain reaction(PCR).E6E7gene,isolated from samples infected with HPV16only,was cloned into plasmid pGEM-3zf and sequenced.Results The recombinant plasmid pGEM/16E6E7was constructed successfully.The whole HPV E6E7gene was776bp in length which was equal to that of the standard strain.Three nucleotides exchanges,i.e.,p60PROE6,p96GLUE6,p565SERE7,were found in E6E7gene.Conclusion The data suggest that there are nucleotide differences of HPV E6E7gene between HPV obtained from Beijing and that of standard sequence.

关 键 词：北京 人乳头瘤病毒16型 E6E7基因 聚合酶链反应 基因变异 

分 类 号：R346[医药卫生—基础医学]