p21与周期蛋白依赖性激酶2在Bowen病皮损中的表达  

Expression of p21and Cyclin-dependent Kinase 2 in Bowen's Disease

作　　者：尤德渊[1] 蔡丽敏[1] 尤海燕[2] 

机构地区：[1]哈尔滨医科大学附属第二医院皮肤科,150086 [2]黑龙江省医院皮肤科

出　　处：《中华皮肤科杂志》2003年第11期635-636,共2页Chinese Journal of Dermatology

基　　金：黑龙江省自然科学基金资助项目(D9708)

摘　　要：目的探讨细胞周期调节蛋白p21及周期蛋白依赖性激酶2(CDK2)在Bowen病中的表达和意义。方法采用链霉亲和素-过氧化物酶法检测28例Bowen病和10例正常人皮肤中p21、CDK2的表达和分布。结果p21与CDK2在Bowen病中均为高表达,而10例正常人皮肤标本中表皮均未见表达。28例Bowen病标本,其中22例(78.6%)呈p21阳性染色,26例(92.9%)呈CDK2阳性染色,两者表达水平呈正相关(r=0.84,P<0.001)。结论Bowen病中肿瘤细胞的增殖能力高于正常细胞,可表达高水平促增殖因子CDK2。p21在Bowen病中的高表达可能与肿瘤细胞的分化有关。Objective To explore the expression and implication of p21and cyclin-dependent kinase2(CDK2)in Bowen's disease.Methods The expression and distribution of p21and CDK2on the skin le-sion and normal skin were detected by using immunohistochemical technique streptavidin-peroxidase(SP)in28cases of Bowen's disease and10normal controls.Results p21and CDK2were significantly highly ex-pressed in Bowen' s disease and were not expressed in normal skins.Positive staining was found in22/28(78.6%)of Bowen' s disease for p21and26/28(92.9%)for CDK2.There was a correlation between the expression of p21and CDK2(r =0.84,P<0.001).Conclusion Significantly higher cellular proliferation is found in Bowen's disease than that in normal skin,and tumor cells overexpress CDK2.Overexpression of p21might be related to the differentiation of the tumor.

关 键 词：BOWEN病 皮肤肿瘤 P21 周期蛋白依赖性激酶2 免疫组织化学 

分 类 号：R739.5[医药卫生—肿瘤]