Cloning, expression, and antiviral activity of interferon β from the Chinese microbat, Myotis davidii  

作　　者：Ying-Zi Liang Li-Jun Wu Qian Zhang Peng Zhou Mei-Niang Wang Xing-Lou Yang Xing-Yi Ge Lin-Fa Wang Zheng-Li Shi 

机构地区：[1]Key Laboratory of Special Pathogens and Biosafety, Wuhan Institute of Virology, Chinese Academy of Sciences [2]Department of Plastic Surgery, Xijing Hospital, Fourth Military Medical University [3]Program in Emerging Infectious Diseases, Duke-National University of Singapore Graduate Medical School

出　　处：《Virologica Sinica》2015年第6期425-432,共8页中国病毒学（英文版）

基　　金：funded by the National Natural Science Foundation of China (No. 31321001)

摘　　要：Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats.Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon(IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β(IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with poly I:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts.Bats are natural reservoir hosts for many viruses that produce no clinical symptoms in bats. Therefore, bats may have evolved effective mechanisms to control viral replication. However, little information is available on bat immune responses to viral infection. Type I interferon (IFN) plays a key role in controlling viral infections. In this study, we report the cloning, expression, and biological activity of interferon β (IFNβ) from the Chinese microbat species, Myotis davidii. We demonstrated the upregulation of IFNB and IFN-stimulated genes in a kidney cell line derived from M. davidii after treatment with polyI:C or infection with Sendai virus. Furthermore, the recombinant IFNβ inhibited vesicular stomatitis virus and bat adenovirus replication in cell lines from two bat species, M. davidii and Rhinolophus sinicus. We provide the first in vitro evidence of IFNβ antiviral activity in microbats, which has important implications for virus interactions with these hosts.

关 键 词：BAT INTERFERON IFN-stimulated genes ANTIVIRAL activity 

分 类 号：S855.3[农业科学—临床兽医学]