南海真菌代谢物1386A对胃癌MCG-803细胞增殖、凋亡和膜电位的影响  

Effects of marine fungal metabolites 1386A from the South China Sea on proliferation,apoptosis and mitochondrial membrane potential in gastric cancer cell line MCG-803

作　　者：陈创奇[1] 方乐堃[1] 刘嘉伟[2] 张剑威[2] 杨广鑫[2] 杨纨[2] 

机构地区：[1]中山大学附属第一医院胃肠外科 [2]中山大学中山医学院,广东广州510080

出　　处：《中国病理生理杂志》2010年第A10期1908-1912,共5页Chinese Journal of Pathophysiology

基　　金：广东省科技计划资助项目(No.2009B030801144);中山大学2007年和2008年"国家大学生创新性实验计划项目";中山大学2010年"广东省大学生创新实验项目"(No.1055810120)

摘　　要：目的:研究南海真菌代谢物1386A对胃癌MCG-803细胞的作用。方法:采用MTT细胞活性检测法检测1386A对MCG-803细胞活性的影响,用集落形成实验检测1386A对MCG-803细胞增殖能力的影响,用流式细胞仪检测1386A对MCG-803细胞周期分布、凋亡、线粒体膜电位的影响。结果:1386A作用于MCG-803细胞24、48和72h后,MTT检测细胞活性的IC50分别为29.70、14.07和13.47μmol/L,1386A作用48h后集落形成实验检测MCG-803细胞增殖的IC50为3.27μmol/L,1386A作用48h后MCG-803细胞的S期比例由25.6%上升为56.8%,1386A作用24h后MCG-803细胞早期凋亡率由3.34%上升为8.39%,1386A作用24h后MCG-803细胞线粒体膜电位下降率为56.26%。结论:南海真菌代谢物1386A能明显抑制胃癌MCG-803细胞的生长,可能通过线粒体凋亡途径诱导细胞的凋亡。AIM:To investigate the effects of marine fungal metabolites 1386A from the South China Sea(1386A)on gastric cancer cell line MCG-803.METHODS:The effects of 1386A on cell viability and cell growth were detected by MTT method and colony-forming assay,respectively.The changes of cell cycle,cell apoptosis and mitochondrial membrane potential were determined by flow cytometry.RESULTS:The IC50 of cell viability on MCG-803 cells after treated with 1386A for 24 h,48 h and 72 h was 29.70 μmol/L,14.07 μmol/L or 13.47 μmol/L,respectively.The IC50 of the cell growth on MCG-803 cells after treated with 1386A for 48 h was 3.27 μmol/L.After treated with 1386A for 48 h,the MCG-803 cells in S stage was increased from 25.6% to 56.8%.After treated with 1386A for 24 h,the early apoptosis of MCG-803 cells was increased from 3.34% to 8.39%,and the mitochondrial membrane potential of MCG-803 cells was decreased by 56.26%.CONCLUSION:1386A has an inhibitory effect on the cell growth in MCG-803 cells through the mitochondrial pathway.

关 键 词：南海真菌代谢物1386A 胃肿瘤 MCG-803细胞 细胞凋亡 线粒体膜电位 

分 类 号：R735.2[医药卫生—肿瘤]