肝癌H-22细胞膜抗原肽提取物对小鼠免疫功能的影响及其抑瘤作用  

Effect of tumor-associated antigen peptide extract from H-22 hepatocarcinoma on immune function and tumor-inhibition in mice

作　　者：孙祖玥[1] 刘淑春[1] 杨英[1] 赵勇[1] 李修义[1] 龚守良[1] 

机构地区：[1]吉林大学卫生部放射生物学重点实验室,长春130021

出　　处：《肿瘤》2004年第1期56-58,共3页Tumor

基　　金：吉林省卫生厅资助项目 (编号 :970 15 )

摘　　要：目的　应用小鼠肝癌H 2 2细胞膜相关抗原肽 (TAP)提取物免疫小鼠 ,观察其对小鼠自身移植肿瘤生长及免疫学参数的影响 ,为制备肿瘤疫苗提供实验依据。方法　采用微酸洗脱法制备分子量≤ 30 0 0Da的细胞膜TAP提取物 ,皮下免疫小鼠 ,检测胸腺细胞增殖反应、T细胞亚组百分数的变化 ,脾细胞ConA反应性、IL 2和IFN γ活性、CTL杀伤活性的变化及抑瘤效应。结果　TAP提取物免疫后 ,移植肿瘤的发生率降低 (P <0 .0 1) ,平均出现时间延迟 (P <0 .0 0 1) ,生长速度减慢 ;同时 ,脾细胞ConA反应性 (P <0 .0 1)、IFN γ(P <0 .0 5 )和IL 2分泌活性和CTL杀伤活性 (P <0 .0 5 )不同程度增强 ;胸腺细胞3 H TdR自发掺入率 (P <0 .0 5 )及CD4 + 和CD8+ T细胞百分数 (P <0 .0 5 )也有不同程度增高。结论　小鼠肝癌H 2 2细胞膜TAP提取物具有免疫原性 ,能有效激发免疫系统功能 ,抑制自身移植肿瘤的生长。Objective To investigate the effect of tumor-associated peptide (TAP) extract on the tumor development in mice autotransplanted with H-22 hepatocarcinoma and the immune functions. Methods Mild acid elution method was applied to prepare TAP extract (MW≤3000 Da) from tumor cell membrane. After immunization with TAP extract, the proliferation activity and the percentage of T cell subsets in thymocytes were detected; the reaction of splenocyte to Con A, the secreting activities of interferon-( (IFN-γ) and interleukin-2 (IL-2) of T cells in spleen were analyzed, and the cytotoxicity of CTL was tested. Results After mice were immunized with TAP extract, the incidence of the transplanted tumor reduced(P<0.01 vs control), the average tumor appearing time delayed(P<0.001), and the growth speed of the tumor decreased in mice. The percentages of CD4 + and CD8 +T cells increased(P<0.05), and the spontaneous incorporation of 3H-TdR into thymocytes increased significantly as compared with that of control after mice were immunized with TAP extract. Meantime, IFN-γ and IL-2 secreting activities increased, and the reaction of the splenocytes to Con A(P<0.01) and the toxic effects of CTL(P<0.05) enhanced significantly. Conclusion The TAP extract has immunogenicity and could efficiently activate immune system and inhibit tumor development. The results suggest that the inhibitory effects of TAP on tumor could be achieved through the enhancement of immune function.

关 键 词：肝癌 H-22细胞膜抗原肽 提取物 免疫功能 抑瘤作用 免疫疗法 

分 类 号：R735.7[医药卫生—肿瘤]