大围山微型鸡和艾维茵肉鸡骨钙含量及CaBP基因表达差异研究  

Study on Bone Calcium Content and CaBP Gene Expression in Daweishan Mini Chicken and Avian Broiler

作　　者：豆腾飞 李涛 闫世雄 黄英[1] 刘丽仙[1] 谷大海[2] 徐志强[2] 李琦华[1] 贾俊静[1] 葛长荣[1] DOU Tengfei;LI Tao;YAN Shixiong;HUANG Ying;LIU Lixian;GU Dahai;XU Zhiqiang;LI Qihua;JIA Junjing;GE Changrong(College of Animal Science and Technology,Yunnan Agricultural University,Yunnan Provincial Key Laboratory of Animal Nutrition and Feed,Kunming,Yunnan 650201;College of Food Science and Technology,Yunnan Agricultural University,Kunming,Yunnan 650201)

机构地区：[1]云南农业大学动物科学技术学院,云南省动物营养与饲料重点实验室,云南昆明650201 [2]云南农业大学食品科学技术学院,云南昆明650201

出　　处：《中国家禽》2019年第2期7-10,共4页China Poultry

基　　金：国家自然科学基金项目(U1702232;31572359;31560618);云南省自然科学基金重点项目(2016FA046);云南省科技厅南希.莱恩院士工作站(2017IC048);云岭学者及云岭产业技术领军人才培养经费;云南省博士研究生学术新人奖

摘　　要：钙结合蛋白(CaBP)可直接影响骨骼中钙的沉积,进而影响骨骼生长发育。为研究家鸡骨钙含量及其CaBP基因表达与骨强度的关系,选用大围山微型鸡和艾维茵肉鸡,分别测定4、8、12周龄时试验鸡的骨强度、骨钙沉积和小肠组织、软骨组织中CaBP基因的相对表达量。结果表明:4、8、12周龄时大围山微型鸡股骨骨强度、骨钙含量均显著高于艾维茵肉鸡(P<0.05),且大围山微型鸡小肠组织、软骨组织中CaBP基因的相对表达量显著高于艾维茵肉鸡(P<0.05)。相关性分析显示,骨钙含量与CaBP基因表达量和骨强度均呈正相关,且CaBP基因表达量和骨强度呈正相关。可见,具有较高的骨钙沉积是大围山微型鸡具有优良骨强度的重要原因,且CaBP基因可作为调控家鸡骨钙沉积的重要候选基因。Calcium binding protein(CaBP) gene can directly affect calcium deposition in bone,which in turn affects bone growth and development.In order to study the relationship between bone calcium content,CaBP gene expression and bone strength in chickens,Daweishan mini chickens and Avian broilers were selected for detecting bone strength,bone calcium content of femur,CaBP gene expression in small intestine and cartilage tissues of chickens at of 4,8and 12 weeks of age.The results showed that the femur bone strength and bone calcium content of Daweishan mini chickens were significantly higher than those of Avian broilers at 4,8 and 12 weeks of age(P<0.05),and CaBP gene expression in small intestine and cartilage of Daweishan mini chickens were significantly higher than that of Avian broilers(P<0.05).Correlation analysis showed that bone calcium content was positively correlated with CaBP gene expression and bone strength,and CaBP gene expression was positively correlated with bone strength.It can be seen that the high bone calcium deposition contributes better bone strength for Daweishan mini chickens,and CaBP gene is an important candidate gene for the regulation of bone calcium deposition in chickens.

关 键 词：大围山微型鸡 艾维茵肉鸡 骨钙含量 CaBP基因 骨强度 

分 类 号：S831.2[农业科学—畜牧学]