禽源大肠杆菌致新生儿大肠杆菌型脑膜炎小鼠模型的构建研究  

Establishment of A Rat Model for Avian Pathogenic Escherichia coli Meningitis

作　　者：刘家奇 武琥琮 尹义 张东 夏芃芃[1,2] 任文凯 朱国强[1,2] LIU Jiaqi;WU Hucong;YIN Yi;ZHANG Dong;XIA Pengpeng;REN Wenkai;ZHU Guoqiang(College of Veterinary Medicine,Yangzhou University,Yangzhou,Jiangsu 225009;Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses,Yangzhou,Jiangsu 225009;Scientific Observation and Experimental Station of Animal Nutrition and Feed Science in South-Central China,Ministry of Agriculture,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Key Laboratory of Agro-Ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,Hunan 410125)

机构地区：[1]扬州大学兽医学院,江苏扬州225009 [2]江苏高校动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009 [3]中国科学院亚热带农业生态研究所畜禽健康养殖研究中心,中国科学院亚热带农业生态过程重点实验室,畜禽养殖污染控制与资源化技术国家工程实验室,湖南省畜禽健康养殖工程技术研究中心,农业部中南动物营养与饲料科学观测实验站,湖南长沙410125

出　　处：《中国家禽》2019年第10期26-30,共5页China Poultry

基　　金：国家重点研发计划项目(2017YFD0500105、2016YFD0500905);国家自然科学基金(31502075、31672579、31270171、31072136、30771603);江苏省自然科学基金(BK20150442);江苏高校优势学科建设工程资助项目

摘　　要：禽致病性大肠杆菌(APEC)临床分离株TW-XM可引起禽类和哺乳动物的脑膜炎,具有感染人类新生儿的潜能并受到医学和学术界极大关注。为探索不同感染剂量的APEC TW-XM感染情况,对新生小鼠进行腹腔接种,通过临床症状观察、存活率差异分析、同一时间点处死发病濒死的小鼠收集样品、脑组织病理学检测、血脑屏障损伤程度及脑膜炎相关炎症因子表达检测等评估,成功构建小鼠脑膜炎模型。基于新生小鼠大肠杆菌型脑膜炎模型建立,为防控大肠杆菌型脑膜炎提供研究基础。Avian pathogenic Escherichia coli(APEC)isolate TW-XM can cause meningitis in both poultry and mammals.APEC strains are capable of causing meningitis in newborn.The aim of this study was to provide research basis for the prevention and control of Escherichia coli meningitis by establishing Escherichia coli caused meningitis model of neonatal ICR mice.Neonatal ICR mice were intraperitoneally inoculated with different does of concentrations of TW-XM.Mice were sacrificed to collect samples at the same time point when they showed clinical symptoms but did not die,clinical symptoms,pathological changes in brain tissues,survival rate difference,damage degree of blood brain barrier and expression of related inflammatory factors were evaluated.Based on the above,the successful establishment of mouse meningitis model for TW-XM laid the foundation of Escherichia coli meningitis.

关 键 词：致脑膜炎型大肠杆菌 新生儿 小鼠脑膜炎模型 

分 类 号：R742.9[医药卫生—神经病学与精神病学] R-332[医药卫生—临床医学]