OK-432抑制人乳腺癌细胞体外增殖及机制研究  

Effect of OK-432 on proliferation of human mammary cancer cells

作　　者：张瑞山[1] 李翔[1] 谢贤鑫 李欢[1] 张腾[1] 马利艳[1] 徐宏[1] 张斌[1] 

机构地区：[1]辽宁省肿瘤医院乳腺外科,辽宁沈阳110042

出　　处：《中国微生态学杂志》2014年第1期31-33,共3页Chinese Journal of Microecology

基　　金：国家自然科学基金资助项目(81102029;81172047)

摘　　要：目的从细胞增殖方面观察OK-432对乳腺癌细胞MCF-7的抑制作用。方法通过MTT比色法、软琼脂克隆形成实验和细胞周期检测观察MCF-7细胞的生长情况;通过流式细胞仪检测MCF-7细胞的增殖细胞核抗原(PC-NA)表达。结果 OK-432能明显降低MCF-7细胞的增殖代谢活性而抑制其增殖。药物处理组具有显著的生长抑制作用(F=3.765,P=0.000)。OK-432组S+G2/M期的细胞比例为(25.53±1.77)%,与空白对照组(39.32±2.56)%相比显著下降(P<0.05)。结论 OK-432能显著抑制MCF-7细胞增殖。Objective To observe the effect of OK-432 on the proliferation of MCF-7 cells. Methods The proliferation inhibitory effect of OK-432 on MCF-7 cells was detected by MTT colormetric assay. FAC was used to identify the expression of PC-NA in MCF-7. Results OK-432 obviously inhibited MCF-7 cells proliferation. The proliferation of MCF-7 cells were significantly depressed in the OK-432 combining group( F = 3. 765,P = 0. 000). The ratio of cells in S + G2 / M stage was significantly lower in OK-432 combining group,( 25. 53 ± 1. 77) % vs( 39. 32 ± 2. 56) %( P < 0. 05). Conclusion OK-432 significantly inhibits the proliferation of MCF-7 cells.

关 键 词：OK-432 乳腺肿瘤 细胞增殖 

分 类 号：R737.9[医药卫生—肿瘤]