益生菌干预对肥胖小鼠肝脏miR-33和miR-122表达的影响  

Effect of probiotic intervention on the expression of miR-33 and miR-122 in the liver of high fat diet induced obese mice

作　　者：唐冬梅[1] 郭艳杰[2] 刘芳[1] 王秋月 杨陈[3] TANG Dongmei;GUO Yanjie;LIU Fang;WANG Qiuyue(Affiliated Zhongshan Hospital of Dalian University,Dalian,Liaoning116001,China;不详)

机构地区：[1]大连大学附属中山医院,辽宁大连116001 [2]大连医科大学,辽宁大连116044 [3]中国农业科学院油料作物研究所,湖北武汉430062

出　　处：《中国微生态学杂志》2019年第9期1005-1008,共4页Chinese Journal of Microecology

基　　金：国家自然科学基金(81601357);大连市科技之星项目(2017RQ040);农业部油料加工重点实验室开放课题(201701)

摘　　要：目的探讨益生菌干预对高脂饮食诱导肥胖小鼠肝脏miR-33和miR-122表达的影响。方法 18只雌性C57BL/6J小鼠随机分为对照组、肥胖组和益生菌干预组,每组6只,分别给予标准饲料、高脂饲料以及高脂饲料+益生菌合剂灌胃,自由采食及饮水,连续喂养6周。每周测量3组小鼠的体质量,6周后,留取小鼠血液样本采用全自动生化仪检测小鼠血脂,安乐法处死小鼠,留取小鼠肝脏样本Hair-pin RT-PCR法检测miR-33和miR-122的含量。结果与对照组小鼠相比,肥胖组小鼠体质量明显增加(t2周=3.985,t3周=4.751,t4周=4.380,t5周=4.728,t6周=4.112,均P<0.01);益生菌干预组小鼠体质量较肥胖组明显降低(t3周=3.694,t4周=4.415,t5周=3.752,t6周=3.392,均P<0.01);肥胖组小鼠血清总胆固醇、低密度脂蛋白含量较对照组明显升高(t=10.850,t=7.024,均P<0.01),益生菌干预组小鼠较肥胖组小鼠血清总胆固醇、低密度脂蛋白含量降低(t=3.034,t=2.881,均P<0.05),但与对照组仍有差异。与对照组小鼠相比,肥胖组小鼠肝脏miR-122的表达升高(t=9.170,P<0.01),miR-33的表达降低(t=3.420,P<0.05),益生菌干预组小鼠较肥胖组小鼠miR-122的表达降低(t=3.204,P<0.05),miR-33的表达升高(t=2.070,P<0.05)。结论益生菌干预能够影响高脂饮食小鼠肝脏miR-33和miR-122的表达,这可能是益生菌干预改善高脂饮食小鼠肝脏脂代谢的机制之一。Objective To explore the influence of probiotic intervention on the expression of miR-33 and miR-122 in the liver of high fat diet induced obese mice.Methods Eighteen female C57 BL/6 J mice were randomly divided into 3 groups:control group(lab chow),obesity group(high fat diet)or probiotic intervention group(high fat diet and probiotics),6 mice in each group.During the experiment,all the mice were weighted weekly.After 6 weeks,the levels of serum lipids were detected by using automatic biochemical analyzer;The levels of miR-33 and miR-122 in mouse livers were detected with Hair-pin RT-PCR method.Results Compared with the control group,the weights in the obese group increased significantly(t2 week=3.985,t3 week=4.751,t4 week=4.380,t5 week=4.728,t6 week=4.112,all P<0.01).The weights in the probiotics intervention group were significantly lower than in the obese group(t3 week=3.694,t4 week=4.415,t5 week=3.752,t6 week=3.392,all P<0.01).The serum levels of total cholesterol and low density lipoprotein in the obese group were significantly higher than those in the control group(t=10.850,t=7.024,all P<0.01),while those in the probiotics intervention group were lower than those in the obese group(t=3.034,t=2.881,all P<0.05),but there were still differences between the two groups.Compared with the control group,the miR-122 level in the obesity group increased(t=9.170,P<0.01),while the miR-33 level decreased(t=3.420,P<0.05).Compared with the obesity group,the expression of miR-122 decreased(t=3.204,P<0.05)and that of miR-33 increased in the probiotics intervention group(t=2.070,P<0.05).Conclusion Probiotics intervention can affect the expression of miR-33 and miR-122 in the liver of high-fat diet mice,which may be one of the mechanisms of probiotics intervention in improving lipid metabolism in the liver of high-fat diet mice.

关 键 词：高脂饮食 肥胖小鼠 益生菌 MICRORNA 

分 类 号：R965[医药卫生—药理学]